代谢工程改造大肠杆菌利用脂肪酸合成己二酸
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(1. 食品科学与技术国家重点实验室,江南大学,江苏 无锡 214122;2. 江南大学 工业生物技术教育部重点实验室,江苏 无锡 214122;3. 江南大学 食品安全国际合作联合实验室,江苏 无锡 214122)

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Q 936

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Metabolic Engineering of Escherichia coli for Synthesis of Adipic Acid from Fatty Acids
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(1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; 2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; 3.International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China)

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    摘要:

    己二酸作为生产尼龙、化纤和工程塑料等聚合物的单体,在食品、医药、塑料和化工行业具有广泛的应用。目前,己二酸的生物合成主要利用可食用原料,碳原子损失较多,造成己二酸产率较低。为解决上述问题,设计和重构以脂肪酸为原料的己二酸合成新路径,提高己二酸的理论得率;其次,强化本源的β-氧化路径和引入异源的ω-氧化路径;增强细胞对脂肪酸的利用能力;再次,借助系统代谢工程策略,进一步优化己二酸合成路径。最后,经发酵条件优化,最优工程菌Escherichia coli AA0304能够产生1.32 g/L己二酸。上述研究结果为利用废弃脂肪酸类化合物生产高价值化学品奠定了良好的基础。

    Abstract:

    Adipic acid, as the monomer for manufacturing polymers such as nylon, fiber and plastics, has broad applications in food, pharmaceutical, plastics, and chemical industries. At present, low-level yield of adipic acid generated from food feedstocks was mainly due to the carbon atom loss. To tackle this issue, an artificial adipic acid biosynthesis pathway for obtaining the highest theoretical adipic acid yield from palmitic acid was designed and constructed. Then, cell utilization for fatty acids was improved by enhancing endogenous β -oxidation pathway and introducing heterogenous ω -oxidation pathway. Furthermore, various strategies of system metabolic engineering were used for further optimizing adipic acid biosynthesis pathway. Finally, the optimal recombinant strain Escherichia coli AA0304 produced 1.32 g/L adipic acid by optimizing fermentation conditions. Therefore, this synthetic platform could lay a good foundation for the production of high-value chemicals from waste oleaginous feedstocks.

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李 洋,闫亚楠,赵春雷,郭 亮,高 聪,刘立明,陈修来.代谢工程改造大肠杆菌利用脂肪酸合成己二酸[J].食品与生物技术学报,2022,41(3).

LI Yang, YAN Yanan, ZHAO Chunlei, GUO Liang, GAO Cong, LIU Liming, CHEN Xiulai. Metabolic Engineering of Escherichia coli for Synthesis of Adipic Acid from Fatty Acids[J]. Journal of Food Science and Biotechnology,2022,41(3).

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  • 在线发布日期: 2022-04-19
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