黄芪多糖通过调节肠道菌群抑制高脂饮食小鼠肠道炎症反应
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(1. 内蒙古医科大学 基础医学院,内蒙古 呼和浩特010110;2. 内蒙古医科大学 护理学院,内蒙古 呼和浩特010110)

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R 285.5

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Experimental Investigation on Inhibitory Effect of Astragalus Polysaccharides on Intestinal Inflammation in High-Fat Diet Mice by Intestinal Flora Regulation
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(1. School of Basic Medicine, Inner Mongolia Medical University, Hohhot 010110, China; 2.School of Nursing, Inner Mongolia Medical University, Hohhot 010110, China)

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    摘要:

    为考察黄芪多糖(APS)通过调节肠道菌群对高脂饮食引起的肠道慢性炎症的治疗作用,选取 30只C57BL/6J小鼠随机分为对照组(C,正常饮食)、模型组(M,高脂饮食)、黄芪多糖处理组(D,高脂饮食并给予2 g/dL黄芪多糖溶液),每组10只,于第11周取血清,剥离脾脏、肾脏周围脂肪组织并称质量。收集小鼠粪便用于16S rRNA高通量测序。ELISA检测血清TC、TG、IgA、IgG、IgM、LPS、IL-1β、IL-6、TNF-α水平。结果表明,APS增加拟杆菌门相对丰度,降低厚壁菌门相对丰度,升高拟杆菌门与厚壁菌门比值,增加双歧杆菌相对丰度,降低脂多糖(LPS)水平,减少炎症因子IL-1β、IL-6、TNF-α的释放,提高脾脏指数,促进IgA、IgG、IgM分泌。由此推测黄芪多糖能够调节肠道菌群结构,增强小鼠免疫功能,抑制LPS引起的肠道炎症。

    Abstract:

    The therapeutic effect of Astragalus polysaccharides (APS) on chronic intestinal inflammation caused by high fat diet through regulating intestinal flora was investigated. Thirty C57BL/6J mice were randomly divided into three groups including the control group (C, fed with normal diet), the model group (M, fed with high fat diet) and APS group (D, fed with high-fat diet and 2 g/dL APS solution), with 10 mice in each group. The serum was collected at week 11, and the adipose tissue around the spleen and kidney was stripped and weighed. The mice faeces were collected for 16S rRNA high-throughput sequencing. The levels of TC, TG, IgA, IgG, IgM, LPS, IL-1β, IL-6 and TNF-α in serum were detected by ELISA. The results showed that APS promoted the abundance of Bacteroidota, decreased the abundance of Firmicutes, increased the ratio of Bacteroidota to Firmicutes, and increased the abundance of Bifidobacterium. It reduced the level of LPS and decreased the release of inflammatory cytokines including IL-1β, IL-6 and TNF-α. It improved spleen index and promoted the secretion of IgA, IgG and IgM. It is thus speculated that APS could regulate the structure of intestinal flora, enhance the immune function of mice, and inhibit LPS-induced intestinal inflammation.

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张竞男,苑 红,马春丽,李 丽,徐宋瑶,张俊锋,霍 达,任贵强,扈瑞平.黄芪多糖通过调节肠道菌群抑制高脂饮食小鼠肠道炎症反应[J].食品与生物技术学报,2022,41(4).

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  • 在线发布日期: 2022-04-29
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