发酵条件对土曲霉菌丝球形态和黄曲霉毒素B1降解效果的影响
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作者单位:

1.河南工业大学粮油食品学院,河南 郑州 450001;2.河南省粮油食品安全检测与控制重点实验室,河南 郑州 450001

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表示共同一作。

通讯作者:

谢岩黎(1971—),女,博士,教授,博士研究生导师,主要从事食品营养与安全研究。E-mail:ylxie@haut.edu.cn

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基金项目:

国家自然科学基金联合基金重点项目(U24A20473);河南省重点研发专项项目(231111113300)。


Effects of Fermentation Conditions on Mycelial Pellet Morphology of Aspergillus terreus and Aflatoxin B1 Degradation Efficency
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Affiliation:

1.College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China;2.Henan Provincial Key Laboratory of Grain, Oil and Food Safety Inspection and Control, Zhengzhou 450001, China

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    摘要:

    目的】探究不同发酵条件对土曲霉(Aspergillus terreus, A. terreus) AterHaut1菌丝球形态和黄曲霉毒素B1 (AFB1)降解效果的影响。【方法】通过单因素试验对不同菌悬液接种体积分数、发酵温度和摇床转速条件下的菌丝球形态和AFB1降解率进行考察,并通过Box-Behnken试验设计结合响应面分析法对发酵条件进行优化。【结果】得到的最佳发酵条件为:转速145 r/min、接种体积分数0.17%、发酵温度35 ℃。在最优条件下,发酵上清液的AFB1实际降解率达96.34%,菌丝球平均直径为1.35 mm,均匀分布在1.20~1.50 mm。【结论】通过优化土曲霉AterHaut1的发酵条件可有效提高AFB1降解率。

    Abstract:

    [Objective] To study the effects of different fermentation conditions on the mycelial pellet morphology of Aspergillus terreus (A. terreus) AterHaut1 and aflatoxin B1 (AFB1) degradation efficency. [Method] Single factor experiments were carried out to investigate the mycelial pellet morphology and AFB1 degradation rates under different inoculation volume fractions, fermentation temperatures, and shaker speeds. The fermentation conditions were optimized by Box-Behnken design combined with response surface methodology. [Result] The optimal fermentation conditions were obtained as follows: shaker speed of 145 r/min, inoculation volume fraction of 0.17%, and fermentation temperature of 35 ℃. The actual degradation rate of AFB1 by the fermentation supernatant obtained under the optimal fermentation conditions reached 96.34%. At this time, the average diameter of mycelial pellets was 1.35 mm, with even distribution between 1.20 mm and 1.50 mm. [Conclusion] Optimizing the fermentation conditions of A. terreus AterHaut1 can improve the degradation rate of AFB1.

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张洪鑫,程思忠,王佳兴,谢岩黎,李倩,孙淑敏,杨玉辉.发酵条件对土曲霉菌丝球形态和黄曲霉毒素B1降解效果的影响[J].食品与生物技术学报,2025,(1):143-153.

ZHANG Hongxin, CHENG Sizhong, WANG Jiaxing, XIE Yanli, LI Qian, SUN Shumin, YANG Yuhui. Effects of Fermentation Conditions on Mycelial Pellet Morphology of Aspergillus terreus and Aflatoxin B1 Degradation Efficency[J]. Journal of Food Science and Biotechnology,2025,(1):143-153.

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  • 收稿日期:2022-05-23
  • 最后修改日期:2022-06-08
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  • 在线发布日期: 2025-05-12
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