Abstract:The phenolic compounds in hemp seed oil can not only improve the antioxidant activity of oil, but also have the functions of anti-cancer, anti-inflammatory, and prevention of chronic diseases such as cardiovascular and cerebrovascular diseases. Among the four oil extraction methods of hot pressing, cold pressing, aqueous enzymatic and leaching, aqueous enzymatic process has the advantages of high total phenolic content and bright color. Therefore, this method is selected for process optimization. In this paper, Alacalase 2.4 L protease was used to hydrolyze hemp seeds and extract oil. The total phenolic content was taken as the optimization index. Three influencing factors were studied by single factor experiment including the amount of enzyme added, the ratio of solid to liquid and the pH value of enzymatic hydrolysis, and the single factor experiment was designed. The optimum process conditions were as follows: enzyme content of 4.57% (mass fraction), the ratio of material to liquid of 1g∶4.79 mL, and pH of 8.24. Under these conditions, the total phenolic content in hemp seed oil was determined to be 65.78 mg/kg. The contribution of various factors to the total phenolic content of hemp seed oil was as follows: the amount of enzyme added > pH value > ratio of solid to liquid. Oxidative damage is an important pathological factor in the development of some malignant diseases and the aging of living organisms. The synchronous growth of wild-type nematodes was treated with H2O2 solution to simulate the oxidative stress damage in nematodes, and the nematode motor deficit model was established. These nematodes were transferred to the culture medium coated with different processed hemp seed oil to investigate the effects of processing methods and main active components in the oil on nematodes. The results showed that the polyphenols in hemp seed oil could improve the behavior of C. elegans, and the order of the improvement effect was as follows: aqueous enzymatic method > hot pressing method > cold pressing method > leaching method.
