磷脂酰-N-乙酰氨基葡萄糖苷的合成及脂质体制备

doi:10.3969/j.issn.1673-1689.2022.06.008

首页 > 过刊浏览>2022年第41卷第6期 >67-75. DOI:10.3969/j.issn.1673-1689.2022.06.008

磷脂酰-N-乙酰氨基葡萄糖苷的合成及脂质体制备
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                        10.3969/j.issn.1673-1689.2022.06.008
                    
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Synthesis and Liposome Preparation of Phosphatidyl-N-Acetylglucosamine

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摘要:

为了提升磷脂酰-N-乙酰氨基葡萄糖苷（PtdGlcNAc）的合成效率，选取不同的咪唑类离子液体，通过以磷脂酶D（phospholipase D，PLD）催化磷脂酰胆碱（phosphatidylcholine，PC）与N-乙酰-D-氨基葡萄糖（N-acetyl-beta-D-glucosamine，GlcNAc）的转酯反应，探究离子液体预处理对酶催化性能的影响。结果表明，经过1-丁基-3-甲基咪唑六氟磷酸盐（[BMIm][PF6]）预处理后，PLD的活性明显高于其他离子液体组和空白组。因此，采用[BMIm][PF6]作为PLD预处理的孵育溶液，通过优化实验，得到最佳反应条件为：1 U的加酶量，底物PC与GlcNAc的物质的量比为1∶60，环戊基甲醚作为有机相，离子液体与水体积比为4∶1，反应时间12 h，产率为79.7%，与未处理组的产率相比提升了56.9%。将合成的PtdGlcNAc制备成纳米脂质体，并对其结构进行了初步表征。该研究丰富了PLD反应体系的相关研究，为其他新型磷脂酰化合物的合成和应用提供了参考。

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The effect of ionic liquid pretreatment on the enzyme catalytic activity was investigated to improve the synthesis efficiency of phosphatidyl-N-acetylglucosamine (PtdGlcNAc) and different imidazole ionic liquids were selected to study the transphosphatidylation of phosphatidylcholine (PC) and N-acetyl-beta-D-glucosamine (GlcNAc) catalyzed by phospholipase D (PLD). The results showed that the enzyme activity of PLD pretreated with 1-butyl-3-methylimidazole hexafluorophosphate ([BMIm][PF6]) was significantly higher than that of other ionic liquids and untreated blank groups. Therefore, [BMIm][PF6] was used as the incubation solution for PLD pretreatment. Through the optimization experiment, the optimum reaction conditions were as follows: 1 U PLD, the amount ratio of substrate PC to GlcNAc of 1∶60, cyclopentyl methyl ether as the organic phase, and the volume ratio of ionic liquid phase to aqueous phase of 4∶1. After 12 h, the product yield was 79.7%, which was 56.9% higher than that of the untreated one. PtdGlcNAc were synthesized into nanoliposomes which structures were preliminary characterized. This study could enrich the related research of PLD and provide a reference for the synthesis and application of other novel phosphatidyl compounds.

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李雪晗,张海洋,刘琦,毛相朝.磷脂酰-N-乙酰氨基葡萄糖苷的合成及脂质体制备[J].食品与生物技术学报,2022,41(6):67-75.

LI Xuehan, ZHANG Haiyang, LIU Qi, MAO Xiangzhao. Synthesis and Liposome Preparation of Phosphatidyl-N-Acetylglucosamine[J]. Journal of Food Science and Biotechnology,2022,41(6):67-75.

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在线发布日期: 2022-06-28
出版日期: 2022-06-25

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