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首页 > 过刊浏览>2024年第43卷第8期 >20-30. DOI:10.12441/spyswjs.20220629001
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汉逊德巴利酵母冻干菌剂制备条件的优化及其对草莓采后软腐病的控制
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国家自然科学基金项目（32372401，32072276）；山东省博士后创新项目（SDCX-ZG-2023-03053）


Optimization of Preparation Conditions of Lyophilized Debaryomyces hansenii Yeast Formulation and Its Control on Postharvest Soft Rot of Strawberry
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    摘要:

    为了更好地利用拮抗酵母菌控制果蔬采后病害，实现酵母制剂的商业化，作者以拮抗酵母菌汉逊德巴利酵母（Debaryomyces hansenii，D. hansenii）为研究对象，使用冷冻干燥技术制备酵母制剂，并研究该制剂对草莓采后软腐病的影响。首先通过单因素试验研究冻干过程中不同保护剂在不同质量分数下对D. hansenii存活率的影响，随后利用响应面法优化保护剂复配方案，最后验证在最优复配方案的保护剂下D. hansenii冻干菌制剂在贮藏期间的存活率及其对草莓采后软腐病的控制效果。结果表明D. hansenii冻干菌制剂的最优保护剂方案为质量分数2.22%的海藻糖、质量分数17.83%的脱脂乳粉、质量分数0.06%的L-抗坏血酸和质量分数4.06%的L-谷氨酸钠。D. hansenii在最优复配方案得到的冻干菌制剂长达90 d的贮藏期内能够保持70%~80%的存活率，且该制剂对由匍枝根霉（Rhizopus stolonifer，R. stolonifer）引起的草莓采后软腐病具有良好的控制效果。

    Abstract:

    The successful use of antagonistic yeasts to control postharvest diseases of fruits and vegetables relies on commercializing yeast preparations. Antagonistic yeast, namely Debaryomyces hansenii (D. hansenii) was used as the research object to analyze the effect of its freeze-dried formulation on the postharvest soft rot of strawberry. Firstly, the impact of different protective agents at different concentrations on the survival rate of D. hansenii in freeze-drying process was examined by a single-factor test. Then, the response surface methodology was used to optimize the protectant regimen. Finally, the survival rate of freeze-dried D. hansenii preparation during storage and its control effect on postharvest soft rot of strawberry was verified under the optimal protectant regimen. The study exposed that the optimal protectant regimen for freeze-drying of D. hansenii preparation was 2.22% alginate, 17.83% skimmed milk powder, 0.06% L-ascorbic acid and 4.06% sodium L-glutamine. D. hansenii in he freeze-dried formulation obtained by the optimal protectant regimen maintained a high survival rate of 70%~80% during prolonged storage of 90 d, and this formulation effectively controlled the postharvest soft rot of strawberry caused by Rhizopus stolonifer (R. stolonifer).

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赵利娜,周娅丽,兰 晨,张晓云,张红印.汉逊德巴利酵母冻干菌剂制备条件的优化及其对草莓采后软腐病的控制[J].食品与生物技术学报,2024,43(8):20-30.

ZHAO Li-na, ZHOU Ya-li, LAN Chen, ZHANG Xiao-yun, ZHANG Hong-yin. Optimization of Preparation Conditions of Lyophilized Debaryomyces hansenii Yeast Formulation and Its Control on Postharvest Soft Rot of Strawberry[J]. Journal of Food Science and Biotechnology,2024,43(8):20-30.

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  • 在线发布日期: 2024-12-11
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