ZIF-8@纤维素酶-细胞共固定化体系的构建及强化丁酸合成的应用

doi:10.3969/j.issn.1673-1689.2022.10.007

首页 > 过刊浏览>2022年第41卷第10期 >77-86. DOI:10.3969/j.issn.1673-1689.2022.10.007

ZIF-8@纤维素酶-细胞共固定化体系的构建及强化丁酸合成的应用
DOI:
                        10.3969/j.issn.1673-1689.2022.10.007
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:Q814.2；TQ921+.4
基金项目:

Construction of ZIF-8@Cellulase-Cell Co-Immobilization System and Its Application in Enhancement of Butyric Acid Synthesis

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

酶-细胞共固定化作为多酶固定化策略的拓展，因其能分层包埋固定化酶和细胞不同生物分子从而强化系统催化性能，成为现阶段酶工程研究的新热点。为了将这一新技术用来强化丁酸的合成，作者基于仿生矿化法构建沸石咪唑酯骨架（zinc zeolitic imidazolate framework-8，ZIF-8）分隔包覆纤维素酶和酪丁酸梭菌（Clostridium tyrobutyricum，Ct），制备ZIF-8@纤维素酶-Ct共固定化体系。通过SEM、FT-IR、XRD等技术表征以及进行酶解、发酵实验的分析验证，该体系具有更优的同步糖化发酵性能。结果表明：在最适条件（40 ℃、pH 4.5）下，底物玉米芯水解率达56.02%，葡萄糖转化率为85.61%；进一步置于不同的pH和氧气体积分数下，发现有ZIF-8@纤维素酶包覆的细胞具有更高、更稳定的比生长率和相对细胞活力以及较低的相对活性氧水平，丁酸在32 h内产量达到6.74 g/L，比未固定化细胞提高了12.52%。

Abstract:

As an extension of multi-enzyme immobilization strategy, enzyme-cell co-immobilization has become a new hot topic in enzyme engineering research because of its ability to enhance the catalytic performance of the system by hierarchically encapsulating different biomolecules of immobilized enzymes and cells. In order to use this new technology to enhance the synthesis of butyric acid, zinc zeolitic imidazolate framework-8@cellulase-Clostridium tyrobutyricum(ZIF-8@cellulase-Ct) co-immobilization system was prepared by constructing ZIF-8 separated with cellulase and Ct based on biomimetic mineralization method. The system was characterized by SEM, FT-IR and XRD techniques and analyzed by enzymatic and fermentation experiments, which showed that the system had better simultaneous saccharification and fermentation performance. 56.02% of corn cobs were hydrolyzed within at 40 ℃ and pH 4.5, and the glucose conversion rate was about 85.61%. Placed under different pH and oxygen concentrations, cells with ZIF-8@cellulase coating were found to have higher and more stable specific growth rate, cell viability and lower relative reactive oxygen levels, and the final concentration of ZIF-8-SSF butyric acid yield reached 6.74 g/L in 32 h, which was 12.52% higher than that of non-immobilized cells.

参考文献

相似文献

引证文献

引用本文

王雨薇,尹成泰,刘婷婷,江凌. ZIF-8@纤维素酶-细胞共固定化体系的构建及强化丁酸合成的应用[J].食品与生物技术学报,2022,41(10):77-86.

WANG Yuwei, YIN Chengtai, LIU Tingting, JIANG Ling. Construction of ZIF-8@Cellulase-Cell Co-Immobilization System and Its Application in Enhancement of Butyric Acid Synthesis[J]. Journal of Food Science and Biotechnology,2022,41(10):77-86.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2022-11-16
出版日期: 2022-10-25

文章二维码

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

文章二维码

微信公众号二维码

手机版网站二维码