Abstract:The aromatic amino acid synthesis pathway in Saccharomyces cerevisiae is very important for the production of many aromatic fine chemicals. Mandelic acid and 4-hydroxymandelic acid like intermediates are vital fine chemicals, which are widely used in foods, medicines, beverages and cosmetics. In order to explore the gene regulation mechanism in aromatic amino acid synthesis pathway in S. cerevisiae to explore the synthesis pathway of aromatic intermediates, this study summarized the whole aromatic amino acid synthesis pathway and its branches from glucose in S. cerevisiae, it also lists 14 genes and intracellular functions involved in the whole process. The phenotypes of 12 single gene deletion strains were detected in sodium dodecyl sulfate (SDS), while 5 deletion strains, including aro2/aro2, aro1/aro1, aro7/aro7, trp1/trp1 and trp5/trp5, showed obvious sensitive phenotypes to 0.03% SDS. Moreover, the intracellular reactive oxygen species (ROS) levels of five sensitive strains were measured. Results showed that the intracellular ROS levels of aro1/aro1, aro7/aro7 and trp1/trp1 strains were significantly increased, compared with the wild-type strain. The whole aromatic amino acid synthesis pathway and its branches in S. cerevisiae were strictly regulated by genes. A systematic study of these genes functions will provide a theoretical basis for a high-yield biosynthesis of aromatic fine chemicals and genomics of S. cerevisiae.
