蛹虫草新菌株ZA10-C4的培养体系优化及虫草素高效制备

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蛹虫草新菌株ZA10-C4的培养体系优化及虫草素高效制备
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基金项目:国家自然科学基金项目（31772373， 32072646， 32272785）；广东省重点领域研发计划项目（2022B0202050002）；广东省现代农业产业技术体系创新团队（食用菌）建设项目（2022KJ103， 2023KJ103）

Cultivation System of a Novel Cordyceps militaris Strain ZA10-C4 and Efficient Preparation of Cordycepin

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摘要:

蛹虫草菌株ZA10-C4是经杂交育种得到的高产虫草素的新菌株，为了获得适合其生长的培养基，提高虫草素产量，作者通过响应面法优化培养基配方，并用大孔树脂NKA-Ⅱ吸附法提取纯化发酵液中的虫草素。ZA10-C4菌株的最佳发酵培养基配方为：麦芽糖17.99 g/L、酵母提取粉41.13 g/L、L-丙氨酸11.83 g/L、KH₂PO₄2.00 g/L、MgSO₄ 1.50 g/L、维生素B₁12 mg/L。在此优化条件下，虫草素产量达到（4 503.14±15.54） mg/L，较优化前提高1.66倍。同时，得到最佳提取纯化工艺为：自然pH、上样体积600 mL、上样和洗脱流量240 mL/h、乙醇（体积分数70%）洗脱体积800 mL。在此条件下得到的虫草素纯度为94.89%，得率为89.57%。该研究为蛹虫草新菌株ZA10-C4的大规模生产及虫草素的高效制备提供了技术参考。

Abstract:

Cordyceps militaris strain ZA10-C4, a newly developed strain through hybrid breeding, exhibits high cordycepin production. In order to obtain a suitable medium formulation for its growth and further improve cordycepin yield, the fermentation medium need to be optimized and the response surface method was applied. Cordycepin was extracted and purified from the fermentation broth using the macroporous resin NKA-II. The optimal fermentation medium formulation was determined to contain maltose17.99 g/L, yeast extract powder 41.13 g/L, L-alanine 11.83 g/L, KH₂PO₄ 2.00 g/L, MgSO₄ 1.50 g/L, and vitamin B₁ 12 mg/L, respectively. Under these optimized conditions, the yield of cordycepin reached (4 503.14±15.54) mg/L, a 1.66-fold increase compared to before optimization levels. Meanwhile, the optimal extraction and purification process was identified as follows: natural pH, a sample loading volume of 600 mL, a sample loading and elution flow rate of 240 mL/h, and ethanol elution with 70% volume fraction at 800 mL. The resulting cordycepin achieved a purity of 94.89% with a yield of 89.57%. This study provides technical reference for the large-scale production of novel Cordyceps militaris strain ZA10-C4 and the efficient preparation of cordycepin.

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麦梦贤,郭丽琼,魏韬,邹苑,郑倩望,叶志伟,林俊芳.蛹虫草新菌株ZA10-C4的培养体系优化及虫草素高效制备[J].食品与生物技术学报,2024,43(7):85-94.

MAI Meng-xian, GUO Li-qiong, WEI Tao, ZOU Yuan, ZHENG Qian-wang, YE Zhi-wei, LIN Jun-fang. Cultivation System of a Novel Cordyceps militaris Strain ZA10-C4 and Efficient Preparation of Cordycepin[J]. Journal of Food Science and Biotechnology,2024,43(7):85-94.

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在线发布日期: 2024-11-13
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