Abstract:This study aimed to use the waste shrimp shell meal as a nitrogen source for the production of single cell protein from Clostridium tyrobutyricum. We investigated the effects of different amount of shrimp shell meal on the growth and production of short-chain fatty acids (acetic acid and butyric acid) of the strain, and determined the content of crude protein, crude fiber, crude fat and the composition of amino acid in the single-cell protein obtained by fermentation of shrimp shell meal by Clostridium tyrobutyricum to comprehensively evaluated the nutritional value of the protein feed. The results showed that the best growth performance was achieved at a carbon to nitrogen ratio of 8∶1.75 between glucose and shrimp shell meal, which produced 1.68 g/L of acetic acid and 3.45 g/L of butyric acid. After the fermentation of shrimp shell meal, the content of crude protein, crude fat and crude fiber increased by 7%, 117% and 92%, respectively, while the content of ash decreased by 13%, compared with those of shrimp shell meal. In addition, the feed contained a rich variety of amino acids, and 12 of the 16 amino acids were increased accordingly compared to the raw material. Finally, combined with the gain of nutritional composition before and after fermentation and the increase of short-chain fatty acids among them, the single-cell protein feed produced by Clostridium tyrobutyricum fermentation of shrimp shell meal is rich in nutritional value, green in production and cheap in cost, which is a novel potential protein feed.
