Abstract:Yeast β-glucan is a type of polysaccharide that is difficult to dissolve in commonly used solvents such as water, acids, and alkalis. Traditional preparation methods are complex, cumbersome, and often result in low product yield or impurities, causing difficulties in industrialization. In this study, an alkaline method combined with dimethyl sulfoxide(DMSO) was used to explore and optimize the production process of yeast β-glucan. The results showed that under the conditions of 0.7 g/dL NaOH at 80 ℃ for 2.6 hour alkaline treatment, the highest impurity removal rate was 71.84%. Separation and purification of β-glucan were accomplished under the conditions of the solid-to-liquid ratio of 30 mg∶1 mL and 80% volume fraction of DMSO at 80 ℃ for 30 minutes, resulting in a β-glucan product with a purity of 95.84%. The product was identified by thin-layer chromatography and Fourier-transform infrared spectroscopy, and the product was indicated as β-glucan composed of glucose monomers. Moreover, Anti-inflammatory experiments showed that this product had a certain biological activity. The study aimed to simplify production processes, scale up production, improve product quality, and provide a theoretical reference for industrialized production.
