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首页 > 过刊浏览>2024年第43卷第10期 >44-53. DOI:10.12441/spyswjs.20230423001
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壳聚糖改性凹凸棒土对食源性致病菌核酸提取效果的影响
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国家“十四五”重点研发计划项目（2022YFF1303305）；江苏省自然科学基金项目（BK20210519）；中国博士后面上项目（2022M723386）；淮安市科技计划项目（HAB202378）。


Extraction Effect of Chitosan Modified Attapulgite on Nucleic Acid of Foodborne Pathogenic Bacteria
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    摘要:

    利用壳聚糖对凹凸棒土（attapulgite，ATP）进行表面改性，考察改性对ATP吸附性能的影响，并建立基于壳聚糖-ATP的DNA提取方法，以实现食源性致病菌基因组DNA的低成本、高效提取。利用X射线衍射光谱等手段对材料进行表征，并利用吸附动力学模型和吸附等温模型考察改性材料对DNA的吸附机制。以3种食源性致病菌基因组DNA为提取对象，考察提取量、纯度、完整性和提取方法的可靠性。结果表明，准一级动力学模型和Freundlich模型能更好地模拟吸附结果，壳聚糖改性可显著提高ATP的吸附性能，壳聚糖-ATP对DNA饱和吸附量为23.648 μg/mg，提取的基因组DNA完整性好、纯度高，且提取方法的重复性好。

    Abstract:

    Surface modification of attapulgite (ATP) using chitosan was conducted to investigate the improvements in the adsorption performance of ATP, and a DNA extraction method based on chitosan-modified ATP was established to achieve low-cost and high-efficiency extraction of genomic DNA from foodborne pathogens. The material was characterized by X-ray diffraction spectroscopy and other techniques, while adsorption kinetics and adsorption isotherm models were used to investigate the adsorption mechanism of modified materials on DNA. Genomic DNA of three foodborne pathogenic bacteria was selected for extraction to investigate the extraction yield, purity, integrity, and stability. The results showed that the experimental data were well-described by the pseudo-first-order kinetic model and Freundlich model. Chitosan modification can significantly improve the adsorption performance of ATP. The saturation adsorption capacity of chitosan-ATP for herring sperm DNA achieved 23.648 μg/mg with high-quality and high-purity, and the chitosan-ATP based extraction method also showed good repeatability.

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王莫凡,彭颖静,李 鹏,成玉梁,于 航,谢云飞,姚卫蓉,郭亚辉.壳聚糖改性凹凸棒土对食源性致病菌核酸提取效果的影响[J].食品与生物技术学报,2024,43(10):44-53.

WANG Mo-fan, PENG Ying-jing, LI Peng, CHENG Yu-liang, YU Hang, XIE Yun-fei, YAO Wei-rong, GUO Ya-hui. Extraction Effect of Chitosan Modified Attapulgite on Nucleic Acid of Foodborne Pathogenic Bacteria[J]. Journal of Food Science and Biotechnology,2024,43(10):44-53.

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  • 在线发布日期: 2025-01-16
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