Abstract:In order to explore the application of supercritical CO2 extraction technology in animal fat extraction and clarify the changes in fatty acid composition during the extraction process, mutton meat was used as the raw material and supercritical CO2 extraction technology was used to extract mutton fat. The extraction effect was analyzed, and the fatty acid mass fraction and composition were analyzed through gas chromatography-mass spectrometry. The results showed that the extraction rate of mutton fat by supercritical CO2 was 22.04%. Compared with the untreated meat, the mass fraction of saturated fatty acid in mutton after supercritical extraction decreased significantly by 11.37% (P<0.05), while there was no significant change in saturated fatty acids in the fat extracted by supercritical extraction. The mass fraction of monounsaturated fatty acids in mutton extracted by supercritical CO2 significantly increased by 9.20% compared to the untreated mutton (P<0.05),of which the mass fraction of palmitoleic acid significantly increased by 61.00% (P<0.05). Compared with the untreated mutton, there was no significant difference in the mass fraction of monounsaturated fatty acids in oil extracted by supercritical CO2 (P>0.05), however, the mass fraction of palmitoleic acid significantly increased by 70.10% (P<0.05). In addition, trans-oleic acid was not detected in both supercritical extraction mutton and fat, and there was no significant change in the mass fraction and composition of polyunsaturated fatty acids (P>0.05). Therefore, the supercritical CO2 extraction technology can be used in the extraction of mutton fat, which is advantageous for increasing the mass fraction of palmitic acid, thus providing a theoretical reference and technical support for the application of supercritical CO2 in animal fat extraction.