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首页 > 过刊浏览>2024年第43卷第6期 >75-85. DOI:10.12441/spyswjs.20231015001
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富硒平菇菌种筛选、发酵优化及菌丝特性评价
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TS 209

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国家“十四五”重点研发项目（2021YFD1600400）


Screening, Fermentation Optimization, and Mycelial Characterization of Selenium-Enriched Pleurotus ostreatus Strains
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    摘要:

    为探究平菇中最优耐硒菌株，确定菌株培养条件及菌丝特性，采用固体培养和液态发酵对4种平菇进行筛选，选取红平菇培养富硒菌丝体，测定其酶活性指标以及菌丝体蛋白质、多糖的体外抗氧化活性。结果表明，红平菇是最佳耐硒菌种，最佳培养条件为：摇床转速150 r/min，pH 6.5，温度25 ℃，添加硒质量浓度5.0 mg/L；富硒菌丝体与普通菌丝体相比，提高了PPO、POD及SOD活性，提高了菌丝抗逆性，且富硒菌丝体及其多糖、蛋白质对DPPH、超氧阴离子自由基和羟基自由基清除能力较强。最后研究了红平菇富硒菌丝体对Caco-2细胞存活率的影响，发现红平菇富硒菌丝体可以抑制Caco-2细胞的存活。该研究表明红平菇富硒液体发酵菌丝体具有较高的生理活性，可作为一种补硒保健品。

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    To explore the optimal selenium-tolerant strains in Pleurotus ostreatus and determine the culture conditions and mycelial characteristics, four kinds of Pleurotus ostreatus were screened by solid culture and liquid fermentation. Pleurotus djamor was selected to culture selenium-enriched mycelium. The enzymatic activity indicators and the in vitro antioxidant activities of mycelial proteins and polysaccharides were determined. The results determined Pleurotus djamor as the best selenium-tolerant species. The optimal culture conditions were as follows: shaking speed of 150 r/min, pH 6.5, temperature of 25 ℃, and selenium concentration of 5.0 mg/L. Compared with the conventional mycelium, the selenium-enriched mycelium promoted the activities of PPO, POD and SOD, improved the stress resistance of mycelium. The selenium-enriched mycelium and its polysaccharides and proteins had strong scavenging ability to DPPH, superoxide anion radical and hydroxyl radicals. Finally, the effect of selenium-enriched mycelium of Pleurotus djamor on the survival rate of Caco-2 cells was investigated, and it was found that the selenium-enriched mycelium could inhibit the survival rate of Caco-2 cells. The selenium-enriched liquid fermented mycelium of Pleurotus djamor has been preliminarily determined with high physiological activity, which could be considered as a selenium supplement.

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张文婧,刘 欢,李红波,莫海珍,刘振彬.富硒平菇菌种筛选、发酵优化及菌丝特性评价[J].食品与生物技术学报,2024,43(6):75-85.

ZHANG Wen-jing, LIU Huan, LI Hong-bo, MO Hai-zhen, LIU Zhen-bin. Screening, Fermentation Optimization, and Mycelial Characterization of Selenium-Enriched Pleurotus ostreatus Strains[J]. Journal of Food Science and Biotechnology,2024,43(6):75-85.

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