Abstract:[Objective] The purpose of this study is to study the antithrombotic effect and composition of peach kernels and provide a theoretical basis for preventing thrombogenesis by peach kernels. [Method] The active components of peach kernels were extracted by ethanol at a volume fraction of 70% and deionized water and separated by multiple chromatography techniques. The antithrombotic components were screened by a rat model of arteriovenous bypass thrombosis and in vitro anti-platelet aggregation test combined with molecular docking. The chemical structures were identified by nuclear magnetic resonance (NMR) spectroscopy. [Result] The 10% ethanol elute of peach kernel water extract separated by MCI column chromatography significantly reduced the thrombus mass in rats (P<0.05). The subsequent separation with ODS column chromatography led to the isolation of five monomer compounds, including mandelate-β-D-glucopyranoside (compound 1), 1-β-D-glucoside phenylidene-2-β-D-glucoside (compound 2), 3-(3-indolyl) butyric acid (compound 3), α-hydroxypropionic acid (compound 4), and dl-mandelic acid (compound 5). Compounds 1, 2, and 5 exhibited inhibitory effects on adenosine diphosphate (ADP)-induced platelet aggregation in vitro (P<0.05). Among them, compound 5 showed the strongest inhibitory effect, with the inhibitory rate reaching 48.67%. [Conclusion] It is proven that dl-mandelic acid is the main active component for the antithrombotic effect of peach kernels.
