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母乳胞外囊泡促进乳双歧杆菌BB-12生长的转录组学分析
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TS201.3

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国家乳业技术创新中心开发课题项目（2023-KFKT-7）


Transcriptomic Analysis of Breast Milk Extracellular Vesicles Promoting the Growth of Bifidobacterium lactis BB-12
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    摘要:

    胞外囊泡是母乳中的重要功能成分之一，作者所在实验室前期研究发现，母乳胞外囊泡能够显著促进乳双歧杆菌BB-12的生长，但具体机制不清楚。作者采用超速离心法与超滤法相结合从母乳中提取胞外囊泡，通过纳米颗粒追踪技术、透射电镜以及蛋白质免疫印迹法对母乳胞外囊泡进行了鉴定表征。利用转录组学技术分析母乳胞外囊泡促进乳双歧杆菌BB-12生长的关键基因。研究发现，添加母乳胞外囊泡后，乳双歧杆菌BB-12菌体中ABC寡肽转运系统相关基因pstC、pstA、metI，淀粉和蔗糖代谢相关基因BIF_02090，磷酸戊糖途径相关基因prsA、BIF_01321，D-氨基酸代谢相关基因murD、BIF_02122，乙醛酸和二羧酸代谢相关基因pccA的表达显著上升。结果表明，母乳胞外囊泡可能作为一种新型益生元促进婴幼儿肠道中双歧杆菌的生长，为母乳胞外囊泡在新一代母乳化奶粉中的开发应用提供了理论依据。

    Abstract:

    Extracellular vesicles are one of the important functional components in breast milk, and previous studies in the author's lab have found that breast milk extracellular vesicles (BM-EVs) could significantly promote the growth of Bifidobacterium lactis BB-12(B. lactis BB-12). However, the specific mechanism is unclear. In this study, BM-EVs were extracted from human milk using the combination of ultracentrifugation and ultrafiltration methods, and were characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and Western-blot analysis. Transcriptome data analysis was used to explore and identify the key genes of BM-EVs promoting the growth of B. lactis BB-12. The results showed that after BM-EVs intervention, the expression levels of ABC oligopeptide transport system-related genes pstC, pstA, metI in B. lactis BB-12 were significantly increased, as well as starch and sucrose metabolism-related genes BIF_02090, pentose phosphate pathway-related genes prsA and BIF_01321, D-amino acid metabolism-related genes murD and BIF_02122, and Glyoxylate and dicarboxylate metabolism- related gene pccA. In summary, BM-EVs may serve as a new type of prebiotic to promote the growth of Bifidobacterium in the intestine of infants，providing a theoretical basis for the development and application in next generation infant formula milk powder.

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李健坤,郑婷,刘伊索,姚昱锟,郝海宁,刘琦琦,易华西.母乳胞外囊泡促进乳双歧杆菌BB-12生长的转录组学分析[J].食品与生物技术学报,2024,43(4):54-61.

LI Jian-kun, ZHENG Ting, LIU Yi-suo, YAO Yu-kun, HAO Hai-ning, LIU Qi-qi, YI Hua-xi. Transcriptomic Analysis of Breast Milk Extracellular Vesicles Promoting the Growth of Bifidobacterium lactis BB-12[J]. Journal of Food Science and Biotechnology,2024,43(4):54-61.

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