鼠李糖乳杆菌利用不同益生元的增殖及耐受性研究
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TS 201.3

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中国食品科学技术学会食品科技基金-雅培食品营养与安全专项科研基金项目(2022-F04)


Proliferation and Tolerance of Lactiplantibacillus rhamnosus Utilizing Different Prebiotics
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    摘要:

    作者旨在通过对比鼠李糖乳杆菌(Lactiplantibacillus rhamnosus)与不同益生元组合后,在生长情况、pH和胆盐耐受性及乳酸产量方面的变化筛选出鼠李糖乳杆菌与益生元的最佳组合。结果表明,鼠李糖乳杆菌GG(L. rhamnosus GG, LGG)、鼠李糖乳杆菌HN001在以低聚半乳糖为唯一碳源生长时,最大生物量分别为1.253、1.552,最大比生长速率分别为0.316、0.290 h-1,乳酸产量分别为3.654、10.914 g/L,与其他益生元组合相比,表现出显著优势。为验证这两种组合在不同胁迫条件下的生长情况,进一步测定了其在不同pH和胆盐质量浓度下的耐受性。这两种组合在1 g/L胆盐条件下的最大生物量分别为0.712和0.694,在pH 4.0条件下的最大生物量分别为0.639和0.728,与其他组合相比存在极显著差异。该研究结果有助于推动鼠李糖乳杆菌与低聚半乳糖组合在食品、医疗等领域的广泛应用,为后续益生菌-益生元组合产品的开发提供了理论依据。

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    To determine the optimal snybiotics of Lactiplantibacillus rhamnosus(LGG) and prebiotics, the LGG-galactooligosaccharide combination is significantly superior to other combinations through growth and pH changes. The maximum biomass OD600 of LGG and HN001 were 1.253 and 1.552, respectively, and the maximum specific growth rates were 0.316 and 0.290 h-1 when galacto- oligosaccharide was used as the sole carbon source. Similarly, the lactic acid yield also showed a significant preference for this combination over others, with yields of 3.654 and 10.914 g/L, respectively. Meanwhile, in order to confirm that the combination can successfully pass through the gastrointestinal tract and exhibit probiotic functions, the tolerance of LGG and HN001 under different pH and bile salt mass concentration was determined respectively when galacto-oligosaccharide was used as the sole carbon source. The results revealed a significant difference in the maximum biomass under 1 g/L bile salt and pH 4.0 conditions compared to other synbiotics, with OD600 values of 0.712, 0.694 (1 g/L bile salt), 0.639, and 0.728 (pH 4.0), respectively. This study provides theoretical basis and experimental evidence for the subsequent development of related probiotic-prebiotic products.

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吴欢欢,宋 馨,夏永,艾连中,潘 澈,毛颖异,李 翔,王光强.鼠李糖乳杆菌利用不同益生元的增殖及耐受性研究[J].食品与生物技术学报,2024,43(4):106-113.

WU Huan-huan, SONG Xin, XIA Yong-jun, AI Lian-zhong, PAN Che, MAO Ying-yi, LI Xiang, WANG Guang-qiang. Proliferation and Tolerance of Lactiplantibacillus rhamnosus Utilizing Different Prebiotics[J]. Journal of Food Science and Biotechnology,2024,43(4):106-113.

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  • 在线发布日期: 2024-05-27
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