Abstract:The study investigated the effects of a compound lactic acid bacteria powder containing Bifidobacterium animalis subsp. lactis XLTG11, Lactobacillus plantarum CCFM8661, 文字Lactobacillus paracasei Glory LP16, and Lactobacillus rhamnosus Glory LG12 on the intestinal microbiota of mice. After adaptive feeding for 7 d, 48 BALB/c mice were randomly divided into negative control, low-, middle-, and high-dose of compound lactic acid bacteria groups. The negative control group received an equal volume of physiological saline via gavage, while the test groups received 5×106, 5×107, and 5×108 CFU of the compound lactic acid bacteria powder, respectively. After 14 d of gavage, the body weight, intestinal microbiota, histopathology, intestinal barrier, intestinal permeability, and short-chain fatty acids (SCFAs) concentration of mice were determined. The results showed that compared with the control group, the feeding of compound lactic acid bacteria significantly increased the body weight of mice and the number of beneficial bacteria such as Lactobacillus and Bifidobacterium in the feces, while significantly decreased the number of Clostridium perfringens. The concentration of short-chain fatty acids increased significantly, accompanied by a significant decrease in LPS and D-lactose. Overall, the compound lactic acid bacteria powder demonstrated the significant improvements in regulating the intestinal microbiota of mice.
