Abstract:To investigate the effect of Lacticaseibacillus rhamnosus Glory LG12 on the intestinal flora of mice, 60 BALB/c mice were acclimatized and fed for 1 week, and then randomly divided into control, model, low-dose, medium-dose, and high-dose groups. The control group was gavaged with an equal volume of saline, while the model group was gavaged with clindamycin and the same volume of saline. The test groups were gavaged with Lacticaseibacillus rhamnosus Glory LG12 at doses of 1.5×106, 1.5×107, and 1.5×108 CFU/mouse, respectively. After 14 d of gavage, determination of body mass, intestinal flora structure, histopathologic changes, intestinal barrier function, intestinal permeability, and short-chain fatty acid concentration in mice. The results showed that compared with the control group, Lacticaseibacillus rhamnosus Glory LG12 significantly increased the number of beneficial bacteria such as Lacticaseibacillus and Bifidobacterium, significantly decreased the number of Clostridium perfringens, and significantly increased in the concentration of short-chain fatty acids in the feces of the mice. Lacticaseibacillus rhamnosus Glory LG12 demonstrated a significant improvement in regulating the intestinal flora of mice.
