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茶树花提取物的体外抗氧化、抗炎活性及其对细胞衰老的抑制作用
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1.云南贝泰妮生物科技集团股份有限公司，云南 昆明 650106;2.上海贝泰妮生物科技有限公司，上海 201702;3.云南特色植物提取实验室有限公司，云南 昆明 650106;4.上海际研生物医药开发有限公司，上海 201702

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云南特色植物筛选与研发服务CXO平台建设项目（2022YKZY001）；云南特色植物新原料自主研发及产业化项目（2023YKZY004）。


In Vitro Antioxidant and Anti-inflammatory Activities of Tea Flower Extract and Its Inhibitory Effect on Cell Aging
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1.Yunnan Botanee Bio-technology Group Co., Ltd., Kunming 650106, China;2.Shanghai Botanee Bio-technology Group Co., Ltd., Shanghai 201702, China;3.Yunnan Characteristic Plant Extraction Laboratory Co., Ltd., Kunming 650106, China;4.Shanghai Jiyan Bio-pharmaceutical Development Co., Ltd., Shanghai 201702, China

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    摘要:

    【目的】 研究云南茶树花提取物（TFE）的体外抗氧化、抗炎活性及其对细胞衰老的抑制作用。 【方法】 通过紫外（UV）-可见分光光度法和高效液相色谱（HPLC）法分析测定TFE的主要活性成分，通过自由基清除试验测定TFE的体外抗氧化能力，利用UV诱导的人永生化表皮角质形成细胞（HaCaT）光老化模型测定TFE的抗炎活性。建立人成纤维细胞衰老模型，通过β-半乳糖苷酶（β-galactosidase，β-gal）染色法检测细胞衰老水平，通过酶联免疫吸附试验检测基质金属蛋白酶-1（matrix metalloproteinase-1，MMP-1）、基质金属蛋白酶-3（matrix metalloproteinase-3，MMP-3）和Ⅰ型前胶原蛋白（type I procollagen，PC-1）基因的表达。 【结果】 TFE中总黄酮、茶多酚、总蛋白质和总糖的质量分数分别为14.51%、12.83%、9.57%、50.34%，其中茶多酚以酯型儿茶素类化合物为主。DPPH、ABTS自由基清除能力的IC50分别为34.32、227.79 μg/mL，TFE显著抑制了UV诱导的HaCaT细胞中活性氧（reactive oxygen species，ROS）的生成，下调了IL-6、TNF-α基因的表达，并上调了核因子E2相关因子2（nuclear factor erythroid-2 related factor 2，Nrf2）和血红素氧合酶-1（heme oxygenase-1，HO-1）基因的表达。此外，TFE还有效抑制了人成纤维细胞的衰老，下调了MMP-1、MMP-3基因的表达，上调了PC-1基因的表达。 【结论】 TFE具有较强的体外抗氧化、抗炎活性，可通过抑制MMP-1和MMP-3基因的表达，提高PC-1基因的表达，来延缓细胞衰老，具有潜在的抗衰老活性。

    Abstract:

    [Objective] This study aims to investigate the in vitro antioxidant and anti-inflammatory activities of Yunnan tea flower extract (TFE) and its Inhibitory effect on cell aging. [Method] The main active components in TFE were determined by ultraviolet (UV)-visible spectrophotometry and high performance liquid chromatography (HPLC). The antioxidant and anti-inflammatory activities of TFE were determined by the free radical scavenging assay and UV-induced photoaging model of human keratinocytes (HaCaT), respectively. After the aging model of fibroblasts was established, the aging level of cells was assessed by the β-galactosidase (β-gal) staining assay, and the expression of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), and type Ⅰ procollagen (PC-1) were determined by enzyme-linked immunosorbent assay. [Result] The mass fractions of total flavonoids, tea polyphenols, total proteins, and total sugars in TFE were 14.51%, 12.83%, 9.57%, and 50.34%, respectively. Ester-type catechins were the main tea polyphenols. The IC50 in scavenging DPPH and ABTS free radicals were 34.32 μg/mL and 227.79 μg/mL, respectively. TFE significantly inhibited reactive oxygen species (ROS) production-induced by UV in HaCaT cells, down-regulated the expression of IL-6 and TNF-α, up-regulated the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). In addition, TFE inhibited the aging of fibroblasts, down-regulated the expression of MMP-1 and MMP-3, and up-regulated the expression of PC-1. [Conclusion] TFE had strong antioxidant and anti-inflammatory activities. It can down-regulated the expression of MMP-1 and MMP-3 and up-regulated the expression of PC-1 to prevent cell aging, demonstrating potential anti-aging activity.

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虞世帅,郝怡宁,彭勃,刘悠云,曲丽萍.茶树花提取物的体外抗氧化、抗炎活性及其对细胞衰老的抑制作用[J].食品与生物技术学报,2025,(7):74-84.

YU Shishuai, HAO Yining, PENG Bo, LIU Youyun, QU Liping.In Vitro Antioxidant and Anti-inflammatory Activities of Tea Flower Extract and Its Inhibitory Effect on Cell Aging[J]. Journal of Food Science and Biotechnology,2025,(7):74-84.

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  • 收稿日期:2024-10-17
  • 最后修改日期:2025-02-17
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  • 在线发布日期: 2025-11-03
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