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首页 > 过刊浏览>2025年第44卷第8期 >97-106. DOI:10.12441/spyswjs.20241207001
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茶花多酚提取工艺优化及其抗氧化、降血糖活性分析
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1.信阳农林学院食品科学与工程学院，河南 信阳 464399;2.河南省大别山特色食物资源综合利用工程技术研究中心，河南 信阳 464399;3.漯河职业技术学院食品技术创新学院，河南 漯河 462002

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朱静（1982—），女，博士，教授，硕士研究生导师，主要从事发酵食品研究。E-mail：zhujingcy@163.com

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基金项目:

河南省重点研发专项项目（231111312800）；河南省重点研发与推广专项（科技攻关）项目（232102230071，242102110090）；河南省高等学校重点科研计划项目（24B550017，24B180016，25B550014）；信阳农林学院科技创新团队项目（XNKJTD-001）。


Extraction Process Optimization and Antioxidant and Blood Glucose-lowering Effect Evaluation of Polyphenols from Camellia japonica Flowers
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1.School of Food Science and Engineering, Xinyang Agriculture and Forestry University, Xinyang 464399, China;2.Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains, Xinyang 464399, China;3.Institute of Food Technology and Innovation, Luohe Vocational Technology College, Luohe 462002, China

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    摘要:

    【目的】优化茶花（Camellia japonica，C. japonica）多酚提取工艺，并初步研究茶花多酚抗氧化、降血糖等功能特性，以提高茶花利用率。【方法】以信阳毛尖茶花为原料，采用超声波辅助法提取茶花多酚，并对其抗氧化、降血糖活性进行分析。以茶花多酚提取率为评价指标，采用响应面法探究乙醇体积分数、料液比、提取时间、超声功率对茶花多酚提取率的影响。【结果】当乙醇体积分数为54%、料液比为1 g∶33 mL、提取时间为49.5 min、超声功率为210 W时，验证试验的实际提取率为12.60%，相对标准偏差（RSD）为2.28%，优化结果可靠。此外，茶花多酚对DPPH自由基、羟基自由基和ABTS+自由基具有较强的清除能力，且该清除能力与茶花多酚质量浓度呈正相关，其半抑制质量浓度（IC50）分别为0.044、0.065、0.068 mg/mL。茶花多酚对α-葡萄糖苷酶和α-淀粉酶的活性有良好的抑制作用，其IC50分别为0.054、0.057 mg/mL。【结论】茶花多酚可作为一种较好的天然抗氧化剂和降糖剂，具有潜在的应用价值，该研究可为茶花精细化加工提供理论依据。

    Abstract:

    [Objective] This study optimized the extraction process of polyphenols from Camellia japonica (C. japonica) flowers and preliminarily explored the antioxidant and blood glucose-lowering effects of the extracted polyphenols, aiming to improve the utilization of C. japonica flowers. [Method] With the flowers of Xinyang Maojian as raw materials, the ultrasonic-assisted method was adopted to extract polyphenols from C. japonica flowers, and the antioxidant and blood glucose-lowering activities of the extracted polyphenols were analyzed. With the extraction rate of polyphenols from C. japonica flowers as the evaluation index, the response surface methodology was adopted to explore the effects of ethanol volume fraction, solid-liquid ratio, extraction time, and ultrasonic power on the extraction rate of polyphenols from C. japonica flowers. [Result] Under the conditions of the ethanol volume fraction of 54%, the solid-liquid ratio of 1 g∶33 mL, the extraction time of 49.5 min, and the ultrasonic power of 210 W, the actual extraction rate obtained from verification experiment was 12.60%, with a relative standard deviation (RSD) of 2.28%, which indicated that the optimization results were reliable. In addition, the polyphenols from C. japonica flowers had strong scavenging abilities against DPPH, hydroxyl, and ABTS+ free radicals. The scavenging abilities were positive correlated with the mass concentration of the extracted polyphenols, and the half-inhibitory mass concentrations (IC50) were 0.044, 0.065, and 0.068 mg/mL, respectively. The extracted polyphenols had good inhibitory effects on the activities of α-glucosidase and α-amylase, with the IC50 of 0.054 and 0.057 mg/mL, respectively. [Conclusion] The polyphenols from C. japonica flowers can be used as a good natural antioxidant and blood glucose-lowering agent, demonstrating potential application value. This study provides a theoretical foundation for the refined processing of C. japonica flowers.

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陈龙,杨晓聪,邢淑婕,史欣窈,郑雪珂,朱静.茶花多酚提取工艺优化及其抗氧化、降血糖活性分析[J].食品与生物技术学报,2025,44(8):97-106.

CHEN Long, YANG Xiaocong, XING Shujie, SHI Xinyao, ZHENG Xueke, ZHU Jing. Extraction Process Optimization and Antioxidant and Blood Glucose-lowering Effect Evaluation of Polyphenols from Camellia japonica Flowers[J]. Journal of Food Science and Biotechnology,2025,44(8):97-106.

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  • 收稿日期:2024-12-07
  • 最后修改日期:2025-02-27
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  • 在线发布日期: 2025-11-26
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