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复合益生菌改善小鼠H1N1流感症状及对肠道菌群和代谢物的影响
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作者单位:

1.国药控股星鲨制药(厦门)有限公司，福建 厦门 361026;2.厦门市母婴健康营养产品重点实验室，福建 厦门 361026;3.江南大学食品学院，江苏 无锡 214122

作者简介:

通讯作者:

王鸿超（1985—），男，博士，副研究员，硕士研究生导师，主要从事肠道菌群与精准营养研究。Email：hcwang@jiangnan.edu.cn

中图分类号:

国家“十四五”重点研发计划项目（2022YFF1100403）。

基金项目:

国家“十四五”重点研发计划项目（2022YFF1100403）。


A Probiotic Formula for Alleviation of H1N1 Infection in Mice and Its Effects on the Gut Microbiota and Metabolites
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Affiliation:

1.Sinopharm Xingsha Pharmaceuticals (Xiamen) Co., Ltd., Xiamen 361026, China;2.Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products, Xiamen 361026, China;3.School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

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    摘要:

    目的 探究复合益生菌在甲型H1N1流感小鼠模型中的缓解效果及其对肠道菌群及代谢物的影响。方法 以具有缓解呼吸道感染能力的Lacticaseibacillus rhamnosus CCFM1279为主，结合Limosilactobacillus reuteri CCFM1145、Lacticaseibacillus casei CCFM1127和多种膳食因子，将3~4周龄SPF级ICR小鼠随机分成4组：空白组、模型组、阳性药物（奥司他韦）组、复合益生菌组，通过鼻滴感染H1N1流感病毒的方式来构建小鼠流感模型。以体质量变化率、病毒载量、肺泡灌洗液中L-乳酸脱氢酶活性等为指标分析复合益生菌缓解甲型H1N1流感症状的能力。采用16S rRNA基因测序评估肠道菌群的变化。采用非靶向代谢组学分析肠道代谢物的变化。结果 口服复合益生菌可有效抵抗甲型H1N1流感，其作用机制在于抑制病毒复制，并减轻肺部炎症损伤，降低肺泡灌洗液中L-乳酸脱氢酶活性。此外，复合益生菌能够调节宿主血清中IL-6、TNF-α质量浓度以及肺部抗病毒蛋白OAS1的相对表达量。复合益生菌显著增加了Parabacteroides的相对丰度。代谢物分析显示，复合益生菌显著提高了丙酮酸等代谢物的相对丰度。结论 甲型H1N1流感小鼠口服复合益生菌后，能够有效抵抗甲型H1N1病毒，并且可能通过特定肠道菌群及代谢物在缓解流感进程中发挥作用，为通过肠道菌群来防治流感的研究提供更多思路。

    Abstract:

    Objective This study aims to explore the alleviating effect of a probiotic formula on H1N1 infection in mice and the effects of this formula on the gut microbiota and metabolites.Method Lactobacillus rhamnosus CCFM1279 with the ability to alleviate respiratory tract infections was combined with Limosilactobacillus reuteri CCFM1145, Lacticaseibacillus casei CCFM1127, and various dietary factors to prepare a probiotic formula. SPF-grade ICR mice of 3 to 4 weeks old were randomized into blank, model, positive control (oseltamivir), and probiotic formula groups. A mouse model of influenza was constructed by nasal inhalation with the H1N1 virus. The alleviating effect of the probiotic formula on H1N1 infection was evaluated based on indicators such as body mass change rate, virus load, and L-lactate dehydrogenase activity in the bronchoalveolar lavage fluid. The 16S rRNA gene sequencing was employed to evaluate changes in the gut microbiota. Non-targeted metabolomics was employed to analyze changes in gut metabolites.Result Oral administration of the probiotic formula effectively resisted H1N1 influenza by inhibiting virus replication, alleviating lung inflammation and damage, and reducing the L-lactate dehydrogenase activity in the bronchoalveolar lavage fluid. In addition, the probiotic formula regulated the mass concentrations of IL-6 and TNF-α in the host serum and the relative expression level of the antiviral protein OAS1 in the lung tissue. It significantly increased the abundance of Parabacteroids. Metabolite analysis showed that the probiotic formula significantly increased the relative abundance of metabolites such as pyruvic acid.Conclusion Oral administration of the probiotic formula can effectively resist the H1N1 virus in the mouse model of H1N1 infection. The probiotic formula may play a role in alleviating influenza progression by specifically regulating the gut microbiota and metabolites. The findings provide new research ideas for the prevention and treatment of influenza via the gut microbiota.

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喻喜华,王一,李刘若兰,赵羽豪,王鸿超.复合益生菌改善小鼠H1N1流感症状及对肠道菌群和代谢物的影响[J].食品与生物技术学报,2025,(4):42-52.

YU Xihua, WANG Yi, LI Liuruolan, ZHAO Yuhao, WANG Hongchao. A Probiotic Formula for Alleviation of H1N1 Infection in Mice and Its Effects on the Gut Microbiota and Metabolites[J]. Journal of Food Science and Biotechnology,2025,(4):42-52.

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  • 收稿日期:2025-01-22
  • 最后修改日期:2025-02-15
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  • 在线发布日期: 2025-08-06
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