复合纳米颗粒负载β-胡萝卜素的Pickering乳液及其酶促降解效应

doi:10.12441/spyswjs.20250808003

首页 > 过刊浏览>2026年第45卷第4期 >94-101. DOI:10.12441/spyswjs.20250808003

复合纳米颗粒负载β-胡萝卜素的Pickering乳液及其酶促降解效应
DOI:
                        10.12441/spyswjs.20250808003
                    
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作者单位:1安徽中烟工业有限责任公司，安徽 合肥 230088;2安徽农业大学食品与营养学院，安徽 合肥 230036;3安徽焦甜香生物科技有限公司，安徽 宣城 241000
作者简介:
通讯作者:杜先锋（1963—），男，博士，教授，博士研究生导师，主要从事食品化学研究。E-mail：dxf@ahau.edu.cn
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基金项目:安徽中烟工业有限责任公司科技项目（202134000034029）。

Pickering Emulsion of Composite Nanoparticle-loaded β-Carotene and Its Enzymatic Degradation Effects

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1China Tobacco Anhui Industrial Co., Ltd., Hefei 230088 China;2School of Food and Nutrition, Anhui Agricultural University, Hefei 230036, China;3Anhui Jiaotianxiang Biotechnology Co., Ltd., Xuancheng 241000, China

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摘要:

【目的】类胡萝卜素裂解双加氧酶（carotenoid cleavage dioxygenase， CCDs）可以用于β-胡萝卜素的酶促降解，但CCDs是亲水性的，因此要促进β-胡萝卜素的酶促降解，就需要一个可以提供双相酶促反应的体系。【方法】制得一种中空介孔二氧化硅（hollow mesoporous silica，HMS）-二氢杨梅素（dihydromyricetin，DMY）复合纳米颗粒（HMS-DMY），用其作为乳化剂构建了负载β-胡萝卜素和CCDs的水包油型Pickering乳液（HMS-DMY-E），分析HMS-DMY的粒径和结构及其对HMS-DMY-E粒径、微观结构和流变性的影响，并分析酶促降解后香气物质的变化。【结果】HMS-DMY的粒径为197.81 nm，多分散指数为0.31，三相接触角为88.14°，显示出良好的界面润湿特性，有助于其在油水界面进行吸附。HMS-DMY-E较小的粒径（21.43 μm）增大了β-胡萝卜素和CCDs的接触面积，促进了β-胡萝卜素的酶解。HMS-DMY-E酶促降解之后，二氢猕猴桃内酯、β-紫罗兰酮和β-环柠檬醛等香气物质的质量分数明显升高。【结论】以构建的Pickering乳液作为微反应容器，可促进CCDs对β-胡萝卜素的酶促降解并提高香气物质的质量分数。

Abstract:

[Objective] Carotenoid cleavage dioxygenases (CCDs) catalyze the degradation of β-carotene. However, due to the hydrophilic nature of CCDs, a system that provides biphasic enzymatic reactions is necessary to facilitate the enzymatic degradation of β-carotene. [Method] Hollow mesoporous silica-dihydromyricetin (HMS-DMY) composite nanoparticles were synthesized and used as the emulsifier to form the oil-in-water Pickering emulsion loaded with β-carotene and CCDs (HMS-DMY-E). The particle size and structure of HMS-DMY were characterized, and their influences on the particle size, microstructure, and rheology of HMS-DMY-E were investigated. Additionally, changes in the content of aroma compounds after enzymatic degradation were analyzed. [Result] HMS-DMY exhibited a particle size of 197.81 nm, a polydispersity index of 0.31, and a three-phase contact angle of 88.14°, showcasing favorable interfacial wettability for oil-water interface adsorption. HMS-DMY-E had a small particle size of 21.43 μm, which enhanced the contact area between β-carotene and CCDs, thereby promoting enzymatic degradation. The mass fractions of key aroma compounds such as β-ionone, β-cyclocitral, and dihydroactinidiolide were significantly increased after enzymatic degradation of HMS-DMY-E. [Conclusion] The Pickering emulsion prepared in this study serves as an efficient microreactor promoting the enzymatic degradation of β-carotene by CCDs and increasing the mass fractions of aroma compounds.

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刁洪林,孙程熠,王文斌,舒俊生,赵伦旭,何召贤,薛雯雯,杜先锋.复合纳米颗粒负载β-胡萝卜素的Pickering乳液及其酶促降解效应[J].食品与生物技术学报,2026,45(4):94-101.

DIAO Honglin, SUN Chengyi, WANG Wenbin, SHU Junsheng, ZHAO Lunxu, HE Zhaoxian, XUE Wenwen, DU Xianfeng. Pickering Emulsion of Composite Nanoparticle-loaded β-Carotene and Its Enzymatic Degradation Effects[J]. Journal of Food Science and Biotechnology,2026,45(4):94-101.

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收稿日期:2025-08-08
最后修改日期:2025-09-28
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在线发布日期: 2026-08-08
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