国产弱阳离子琼脂糖交换介质的性能及其用于蛋清溶菌酶的分离纯化

doi:10.3969/j.issn.1673-1689.2009.06.016

首页 > 过刊浏览>2009年第28卷第6期 >799-803. DOI:10.3969/j.issn.1673-1689.2009.06.016

国产弱阳离子琼脂糖交换介质的性能及其用于蛋清溶菌酶的分离纯化
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                        10.3969/j.issn.1673-1689.2009.06.016
                    
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基金项目:国家自然科学基金项目(20676053);国家863计划项目(2007AA02Z207)

Characteristics and Performances of National Agrose Cation Exchanger and Its Application in Lysozyme Purification from Egg White

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从理化性能、离子交换容量、静态和动态吸附性能等方面对国产快流速CM-琼脂糖弱酸性阳离子交换介质进行了综合考察和评价,并应用该介质进行了鸡蛋清中溶菌酶的分离。结果发现,国产介质的理化性能符合要求,离子交换容量达到了187 mmol/mL,高于国外商品介质;对模型蛋白溶菌酶的静态和动态吸附容量分别达到了107.0 mg/mL和90.3 mg/mL(高流速下),同样略高于国外商品介质,显示能够满足蛋白质的有效吸附;离子交换层析分离蛋清溶菌酶较为成功,纯度在95%以上,产量达到了4.1 mg/mL鸡蛋清。

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The national cation exchanger(Zhengguang CM-Agrose Fast Flow) was integrated evaluated on its physic properties,functional group density,static and dynamic adsorption properties.Besides,the adsorbents were used to purification of lysozyme from egg white.As a result,it was found that the physic properties of CM-Agrose FF include density,water content and size distribution are up to the requirement.The functional group density reaches 187 mmol/mL,which is higher than CM Sepharose FF.The static and dynamic adsorption of lysozyme reach 107.0 and 90.3 mg/mL(100 cm/h),respectively,which indicated that the adsorbents could fulfil the efficient adsorption of proteins.Lysozyme was successfully purified from egg white using the cation exchanger.The purity of lysozyme was more than 95% and the yield reached 4.1 mg/mL egg white.

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夏海锋,金雄华,饶志明,郝飞,严伟,郑志永.国产弱阳离子琼脂糖交换介质的性能及其用于蛋清溶菌酶的分离纯化[J].食品与生物技术学报,2009,28(6):799-803.

XIA Hai-feng, JIN Xiong-hua, RAO Zhi-ming, HAO Fei, YAN Wei, ZHENG Zhi-yong. Characteristics and Performances of National Agrose Cation Exchanger and Its Application in Lysozyme Purification from Egg White[J]. Journal of Food Science and Biotechnology,2009,28(6):799-803.

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在线发布日期: 2014-06-16
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