Abstract:The immobilization conditions,and application acid urease via adsorption and embedded were investigated in this study.The optimum concentration of sodium alginate,calcium chloride and chitosan was 2.0,5.0 and 3.0 g/dL,respectively.the optimal immobilized time was 2.5h,the immobilization rate was 82.3%,and the reaction take place at 40℃and pH4.The half-lives of immobilized acid urease was 48 days,the unit of immobilized urease was 3.6U/mg,the urea removal rate was 65% at 35℃,24 h and the addition level of immobilized acid urease was 0.1g/mL (the quality of immobilized enzyme /the volume of Chinese rice wine),under the same conditions,the urea removal rate still reached at 39.7% under the continuous using thirteen times.
