Abstract:In this manuscript,response surface design was used to optimize the nutritional and environmental conditions of cellulase production by Penicillium glaucum NS16.There are 18 factors affect the cellulose production,including the fermentation time,shaking rate,concentration of carbon sources,nitrogen sources and inorganic salts.The influence of the 18 factors was evaluated by Plackett-Burman design.The result showed that wheat bran,CMC-Na and the fermentation time play important roles on the cellulase production.According to the result of Plackett-Burman design experiment,the value range of the significant influential factors could be estimated,and a Box-Behnken design was employed to optimize these factors and the results were shown in response surface graph.Following the results,the optimum conditions listed as follows:6.0 g/L wheat bran,7.0 g/L CMC-Na,and 96 hours feimentation time.By the optimum conditions,309 IU/ml of cellulase was produced by Penicillium glaucum NS16,which was higher 227% than that of the control (94.51 IU/mL)
