Abstract:In this manuscript,a novel ultra performance liquid chromatography(UPLC) coupled with mass spectrometry(MS) method was developed for the simultaneous determination of six micro-sweeteners(acesulfame-K,saccharin sodium,sodium cyclamate,sucralose,aspartame,neotame) in distilled spirit.After sample preparation,the analytes was seperated on an BEH C-18 column with methanol-20 mM ammonium acetate as mobile phase for gradient elution and detected at electrospray ionisation(ESI) source operating in negative mode,along with the accurate mass of their main fragment ion.It takes only 10 minutes to finish a whole seperation and detection.Under the optimized conditons the six compounds behave linearly in the 0.05-5.0 mg/L concentration range,with correlation coefficient >0.9945.The detection limits is 0.05 mg/L for acesulfame-K,0.01 mg/L for saccharin sodium,sodium cyclamate,neotame and 0.1 mg/L for sucralose,aspartame.The recoveries at the tested concentrations of 0.5 and 1.0 mg/L were 86.48 %~116.90 %,with the relative standard deviations of 0.22 %~3.90 %.The method has been successfully applied to simultancously detect six synthetic sweeteners in distilled spirit samples and illustrated accurate and effective.