Abstract:To investigate the anoxia endurance and immunological of mung bean protein or peptide(MBPP) in mice.This research employs high performance gel permeation Chromatography(HPGPC)to investigate and evaluate the molecular weight’s(Mw)distribution of MBPP.The tested mice were randomly distributed into 3 groups,and were continuously given MBPP by intragastric administration for 30 days,then the living time of mice were measured in anoxia with normal air pressure,in poisoning by nitrite poisoning and in acute lack of blood in brain.The effect was evaluated on celluar immunity by ConA lymphocyte transformation test(MTT) and delayed type hypersensitivity(DTH).The impact of humoral immunity was detected by antibody-producing cells(PFC) test and the value of half of hemolysis(HC50) in mice.The non-specific immunity was detected by abdomen macrophages of mice devour chicken red cell test.The Mw MBPP is between 195~1000D,in which the component of 195-510D takes 70.2% or above.MBPP of 0.82 g/kg·BW and 1.23 g/kg·BW.dose group could significantly prolong the living time of mice with hypoxia(P<0.05) and long-nitrite poisoning in mice living time(P<0.05).Compared with that of the control group,MBPP could promote DTH,PFC,HC50 and Macrophage phagocytosis ability in mice.MBPP could improve the anoxia endurance and immunological in mice.
