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高效转化L-谷氨酸为γ-氨基丁酸菌株的筛选、鉴定及初步优化
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国家自然科学基金项目(20676053,30970056);国家863计划项目(2006AA020103,2007AA02Z207)


Screening,Identification and Primary Optimizing of a Strain Producing γ-Aminobutyric Acid from L-Glutamic Acid
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    摘要:

    从发酵食品及自然界中筛选出能利用L-谷氨酸生产γ-氨基丁酸的菌株,并对转化条件进行初步优化。采用乳酸菌分离纯化的方法分离出乳酸菌,再通过初筛、复筛挑选出高产γ-氨基丁酸的菌株,并对其进行形态学、常规生理生化鉴定及16S rDNA基因分析,随后对菌株产γ-氨基丁酸的转化条件进行初步优化。根据常见细菌系统鉴定手册,所筛菌株初步确定为植物乳杆菌(Lactobacillus plantarum)。优化后的最佳转化条件为:菌株菌龄为36 h、缓冲液初始pH为4.8、缓冲液浓度为0.2 mol/L。在优化后的转化条件下,添加5 g/dL底物转化24 h,转化率最高可达97.81%,转化液中γ-氨基丁酸的质量浓度达34.26 g/L,该菌种可作为γ-氨基丁酸产生菌,具有较好的γ-氨基丁酸生产潜力。

    Abstract:

    A strain with capablity of producing γ-Aminobutyric acid from L-glutamic acid was isolated from variety of fermented food and different natutal environment samples,and the conditions of transformation were optimized.Process and Method: Isolation and purification method of Lactic acid bacteria was used to screen the strain yielding γ-amino butyric acid,and its morphology analysis,physiological and biochemical properties and phylogenetic analysis based on the 16S rDNA sequence were identified.And then the conditions of transformation were optimized for the Lactic acid bacteria separately.Based on Systems Manual of Determinative Common Bacteriology,the bacteria was preliminarily identified Lactobacillus plantarum.The better transformation conditions as follows: cell age 36 h,buffer initial pH 4.8,buffer concentration 0.2 mol/L;The content of GABA was over 34.26 g/L and transformation efficient was up to 97.81% in the optimum transformation conditions.The strain can be used to produce γ-amino butyric acid and has a higher converting capability of GABA.

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刘婷婷,杨套伟,张术聪,夏海锋,饶志明.高效转化L-谷氨酸为γ-氨基丁酸菌株的筛选、鉴定及初步优化[J].食品与生物技术学报,2010,29(5):742-747.

LIU Ting-ting, YANG Tao-wei, ZHANG Shu-cong, XIA Hai-feng, RAO Zhi-ming. Screening, Identification and Primary Optimizing of a Strain Producing γ-Aminobutyric Acid from L-Glutamic Acid[J]. Journal of Food Science and Biotechnology,2010,29(5):742-747.

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