大孔树脂纯化笋壳中的黄酮物质
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贵州省科技支撑计划项目(黔科合NY字[2009]3028)


Purification of Flavonoids from Bamboo Shell by Macroprous Absorbent Resins
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    摘要:

    通过比较5种大孔吸附树脂对笋壳黄酮的吸附分离性能,筛选出适合分离笋壳黄酮的树脂,并对其动态吸附特性进行研究。结果表明:HPD600树脂对笋壳黄酮不仅吸附量大,而且解吸率高,适合笋壳黄酮的分离富集。其分离笋壳黄酮的工艺参数为:上样质量浓度为3.89 mg/mL,pH 3.0,上样量为7 BV,流速3 BV/h;用6 BV的体积分数40%乙醇洗脱,解吸效果最佳,黄酮总回收率为82.33%,可得总黄酮质量分数为35.12%的笋壳提取物粉末。

    Abstract:

    In this manuscript,five types of macroporous resins were selected for adsorption and separation of flavonoids from Bamboo Shell.It was found that resin HPD600 was an excellent adsorbent with higher adsorption and desorption capacity.The effects of concentration,pH and flow rate of the solution on the dynamic adsorption of resin HPD600 were also studied.The results showed that the optimum adsorption conditions are listed as following: 7 BV flavonoids concentration of extract solution at 3.89 mg/mL,pH 3.0,flow rate at 3 BV/h using 6 BV40% ethanol as the best desorption solvent,the total recovery of chlorogenic acid reached at 82.33%,and the content of flavonoids reached at 35.12% in the crude product.

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杨乐,王洪新,秦晓娟.大孔树脂纯化笋壳中的黄酮物质[J].食品与生物技术学报,2011,30(2):218-223.

YANG Le, WANG Hong-xin, QIN Xiao-juan. Purification of Flavonoids from Bamboo Shell by Macroprous Absorbent Resins[J]. Journal of Food Science and Biotechnology,2011,30(2):218-223.

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  • 在线发布日期: 2014-06-17
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