费约果叶片总黄酮提取工艺优化及其抗氧化活性研究

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费约果叶片总黄酮提取工艺优化及其抗氧化活性研究
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基金项目:四川省科技厅重点项目(07zs2106);西南科技大学博士基金项目(08zx7104)

Optimization of Extraction Process and Antioxidant Activities of the Total Flavonoids from Feijoa sellowiana Leaves

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摘要:

对费约果叶片总黄酮的提取工艺和抗氧化活性进行了研究。结果表明:费约果叶片总黄酮最佳提取工艺为温度50℃、料液比1∶30(g∶mL)、提取时间50 min和甲醇体积分数70%。根据最佳提取工艺,重复3次得到总黄酮量分别46.89、44.51、48.27 mg/g,具有稳定性和可操作性;另外,通过半抑制浓度IC50衡量费约果叶片总黄酮提取物抗氧化活性,其抗脂质过氧化能力(IC500.275 mg/mL)和DPPH.自由基的清除能力(IC500.798 mg/mL)均优于抗坏血酸(IC500.643、IC500.917 mg/mL),而超氧阴离子自由基的清除能力(IC500.774 mg/mL)和羟自由基的清除能力(IC500.278 mg/mL)均弱于抗坏血酸(IC500.537I、C500.275 mg/mL)。

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This manuscript optimized the extraction technologies and investigated the antioxidant activities of the total flavonoids from Feijoa sellowiana leaves.The results determined that the optimum parameters of extraction process as follows: the extraction temperature 40 ℃,the ratio of sample to extraction solution 1:30,the extraction time 50 min and the concentration methyl alcohol 70%.With the optimum extraction parameters,the total flavonoids contents with three reiterations achieved at 46.89 mg/gFW,44.51 mg/gFW and 48.27mg/gFW,respectively.In addition,half-inhibitory concentration IC50 was used to evaluate antioxidant activities of total flavonoids from Feijoa leaves.The capability of inhibiting lipid peroxidation(IC500.275 mg/mL) and scavenging effects on DPPH freedom radical(IC500.798 mg/mL) of total flavonoids extracts were stronger than the corresponding values of ascorbic acid(IC500.643 mg/mL and IC500.917 mg/mL).However,the scavenging effects on superoxide anion(IC500.774 mg/mL) and hydroxyl freedom radical(IC500.278 mg/mL) were weaker than that of ascorbic acid(IC500.537 mg/mL and IC500.275 mg/mL).

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丁振柱,黄仁华,王丹.费约果叶片总黄酮提取工艺优化及其抗氧化活性研究[J].食品与生物技术学报,2011,30(3):371-375.

DING Zhen-zhu, HUANG Ren-hua, WANG Dan. Optimization of Extraction Process and Antioxidant Activities of the Total Flavonoids from Feijoa sellowiana Leaves[J]. Journal of Food Science and Biotechnology,2011,30(3):371-375.

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