Aspergillus ficuum内切菊粉酶基因在大肠杆菌中表达
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

食品科学与技术国家重点实验室2008年度目标导向项目(SKLF-MB-200804);江苏省自然科学基金创新学者攀登项目(BK2008003)


Expression of Endo-Inulinase gene from Aspergillus ficuum in Escherichia coli
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    研究内切菊粉酶基因在大肠杆菌中表达,利用基因工程的原理和方法,将来源于Asper-gillus ficuum JNSP5-06的内切菊粉酶基因(endoI)克隆到pET-28a(+),并在大肠杆菌BL21(DE3)中进行表达。重组菌经IPTG诱导后对表达产物进行酶活检测和酶水解产物分析。结果表明已成功构建表达载体pET28a-endoI,表达后的粗酶活为35 U/mL发酵液;重组酶水解菊粉的产物经TLC分析证实酶液具有内切菊粉酶活性,水解产物主要为低聚二糖、三糖和四糖。为其应用和工业化生产奠定基础。

    Abstract:

    In order to expand the industrial application of endo-inulinase,its gene from Aspergillus ficuum JNSP5-06 was cloned into the pET-28a(+) and expressed in E.coli BL21(DE3) and get a mutant strain.The mutant was induced by IPTG before the hydrolysis activity was measured and the product was analyzed.The expression vector pET28a-endoI was successfully obtained and the crude enzyme activity was 35 U/mL(fermentation broth).The hydrolysis product from the recombinant inulinase was identified by TLC analysis,indicating that endo-inulinase enzyme solution had activity and the major products were disaccharide,trisaccharide,and tetrasaccharide.These results suggested that the endo-inulinase gene has been successfully expressed in E.coli and significantly improved the endo-inulinase activity.

    参考文献
    相似文献
    引证文献
引用本文

陈晓明,陈静,陈寒青,金征宇,徐学明. Aspergillus ficuum内切菊粉酶基因在大肠杆菌中表达[J].食品与生物技术学报,2011,30(3):388-393.

CHEN Xiao-ming, CHEN Jing, CHEN Han-qing, JIN Zheng-yu, XU Xue-ming. Expression of Endo-Inulinase gene from Aspergillus ficuum in Escherichia coli[J]. Journal of Food Science and Biotechnology,2011,30(3):388-393.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2014-06-17
  • 出版日期:

版权所有:《食品与生物技术学报》编辑部

地址:江苏省无锡市蠡湖大道1800号  邮政编码:214122

电话:0510-85913526  电子邮件:xbbjb@jiangnan.edu.cn

技术支持:北京勤云科技发展有限公司

微信公众号二维码

手机版网站二维码