低聚壳聚糖与葡萄糖和麦芽糖的美拉德反应及其衍生物的抗氧化性能

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低聚壳聚糖与葡萄糖和麦芽糖的美拉德反应及其衍生物的抗氧化性能
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基金项目:上海市生物医药和农业科技领域重点科技项目(08391911500);2009年上海市优秀学科带头人计划(09XD1402000);上海市教委重点学科建设项目(J50704)

Maillard Reaction between Chitosan Oligosaccharide and Glucose/Maltose and the Antioxidant Activity of the Derivatives

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低聚壳聚糖(COS)作为氨基供体分别与提供羰基的葡萄糖和麦芽糖进行美拉德反应(氨基与羰基的物质量比均为3:1),考察了反应过程中吸光度和荧光值的变化。醇沉法提取低聚壳聚糖美拉德反应衍生物CG和CM。对两种衍生物进行红外表征和相对分子质量测定,并研究其对超氧阳离子O2.-、DPPH自由基的清除能力以及还原能力。结果显示:抗氧化能力强弱次序为CG>CM>COS,即美拉德反应后低聚壳聚糖衍生物抗氧化能力得到显著提高,且CG的抗氧化活性优于CM,表明与单糖进行美拉德反应制得的壳聚糖衍生物具有更好的抗氧化性。

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Maillard reaction was occured by heating chitosan oligosaccharide(COS) with glucose and maltose separately(the ratio of —NH2 and —OH was 3∶1).The absorbance and fluorescence were determined during the reaction.Two kinds of chitosan oligosaccharide derivatives were extracted,named CG and CM.Chitosan oligosaccharide derivatives were characterized by FTIR and their molecular weight was determined by GPC.Their antioxidant activity was investigated by employing various established in vitro systems,such as superoxide/1,1-diphenyl-2-picrylhydrazyl(DPPH) radicals scavenging and reducing power.The results showed two kinds of the chitosan oligosaccharide derivatives showed strong antioxidant activity compared with chitosan oligosaccharide.The antioxidant activity was found to be in the order of CG>CM>COS,which indicated that the derivatives prepared by monosaccharide had the better antioxidant activity.

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孙涛,陈春红,谢晶,梁齐.低聚壳聚糖与葡萄糖和麦芽糖的美拉德反应及其衍生物的抗氧化性能[J].食品与生物技术学报,2011,30(4):556-560.

SUN Tao, CHEN Chun-hong, XIE Jing, LIANG Qi. Maillard Reaction between Chitosan Oligosaccharide and Glucose/Maltose and the Antioxidant Activity of the Derivatives[J]. Journal of Food Science and Biotechnology,2011,30(4):556-560.

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在线发布日期: 2014-06-17
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