L-缬氨酸产生菌的原生质体融合及抗高糖融合株的筛选

首页 > 过刊浏览>2011年第30卷第4期 >597-601

L-缬氨酸产生菌的原生质体融合及抗高糖融合株的筛选
DOI:
                        
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:国家863计划项目(2008AA02Z212)

Protoplast Fusion of L-Valine Producer and Screening of Fusants by Improving High-Glucose Tolerance

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

通过原生质体融合技术选育出一株抗高糖的融合株。以黄色短杆菌(Brevibacterium flavum)NV128和黄色短杆菌(B.flavum)JV16(Leu-Ile-Met-)为亲本菌株,通过单灭活原生质体融合、高糖梯度平板筛选及进一步的硫酸二乙酯(DES)诱变,获得了一株L-缬氨酸高产菌NJ-237。同时研究了影响原生质体形成、再生的3种重要条件(青霉素、酶和渗透压稳定剂),并确定了其最佳处理浓度和时间。该融合株经7 L发酵罐培养72 h L-缬氨酸达到46.8 g/L,糖酸转化率为27.7%,比两亲株都有显著提高。

Abstract:

A fusant which could be resistant to high concentration of glucose was screened by fusing protoplasts in this study.The L-valine overproducing fusant was derived from Brevibacterium flavum NV128 and JV16(Leu-Ile-Met-),and was screened on high-glucose gradient plates by means of fusing the single inactivated protoplasts and diethylsulfate(DES) treatment.At the same time,three different conditions(penicillin,lysozyme and osmotic stabilizer)affecting the formation and regeneration of protoplasts were studied and the optimum conditions were determined.Under the optimum conditions,the titer of L-valine achieved at 46.8g/L after 72h culture,and the yield of L-valine on glucose was 27.7%.

参考文献

相似文献

引证文献

引用本文

袁丹丹,张伟国. L-缬氨酸产生菌的原生质体融合及抗高糖融合株的筛选[J].食品与生物技术学报,2011,30(4):597-601.

YUAN Dan-dan, ZHANG Wei-guo. Protoplast Fusion of L-Valine Producer and Screening of Fusants by Improving High-Glucose Tolerance[J]. Journal of Food Science and Biotechnology,2011,30(4):597-601.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2014-06-17
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码