黄粉虫多糖响应面法提取及抗氧化活性

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黄粉虫多糖响应面法提取及抗氧化活性
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基金项目:国家林业局引进国外先进农业技术项目(20064116)

Optimization of Extraction Process by Using Response Surface Methodology and Antioxidant Activity of Polysaccharide from Yellow Mealworm

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采用响应面分析法研究黄粉虫多糖提取的工艺条件,同时检测提取多糖的抗氧化活性。通过对关键影响因子的最佳水平范围的研究,依据回归分析确定了黄粉虫多糖最佳提取条件为:提取温度90℃、液固比12、提取时间158 min,在此条件下黄粉虫多糖提取率的理论值为1.99%,实测值为1.94%。分析表明,黄粉虫多糖主要由葡萄糖组成,并含少量甘露糖和半乳糖。体外抗氧化试验显示,黄粉虫多糖具有一定的抗氧化作用,可作为潜在的天然抗氧化剂来源。

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In this study,respone surface analysis methodology(RSM) was applied to optimize the extraction conditions of polysaccharide from yellow mealworm(Terebrio molitor L.).Antioxidant capability of the polysaccharide was studied in vitro.The optimal range of key factors were studied by regression analysis and listed as follows: temperature 90 ℃,time 158 min,the ratio of liquid to solid 12.With the optimum conditions,the yield of polysaccharide was achieved at 1.94%(the predicted yield was 1.99%).The polysaccharide from yellow mealworm was constituted glucose mainly,mannose and galactose.The results also demonstrated that yellow mealworm polysaccharide has antioxidant activity in vitro,which means it could be explored as a potential natural antioxidant.

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何钊,冯颖,孙龙,陈智勇,陈晓鸣.黄粉虫多糖响应面法提取及抗氧化活性[J].食品与生物技术学报,2011,30(5):641-647.

HE Zhao, FENG Ying, SUN Long, CHENG Zhi-yong, CHENG Xiao-ming. Optimization of Extraction Process by Using Response Surface Methodology and Antioxidant Activity of Polysaccharide from Yellow Mealworm[J]. Journal of Food Science and Biotechnology,2011,30(5):641-647.

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在线发布日期: 2014-06-17
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