Abstract:Based on the lipase sequences from 34 kinds of strain,the relation between amino acids and dipeptides with the optimum temperature were built by a stepwise regression method.The results showed that the positively correlated amino acid was Y,while the negatively correlated ones were I,S and K,and that the positive dipeptides were IR,KS,NY,SA,ST,YR,whereas the negative ones were DK,DY,IS,KA,WS,YS,QI.Through analysis of position information of the related dipeptides in thermophilic and mesophilic lipases,it was found that most positive dipeptides were sited in the α-helix and on the surface of lipases,whereas most negative ones were located in the β-strand or coil,which distributed in buried regions of lipases and often existed in the N or C terminus of lipase chains.
