发酵液中威兰胶的乙醇沉淀行为

doi:10.3969/j.issn.1673-1689.2012.03.006

首页 > 过刊浏览>2012年第31卷第3期 >259-265. DOI:10.3969/j.issn.1673-1689.2012.03.006

发酵液中威兰胶的乙醇沉淀行为
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                        10.3969/j.issn.1673-1689.2012.03.006
                    
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基金项目:国家863计划项目(2006AA02Z207);国家自然科学基金项目(20676055,20806034)

Recovery Characteristics of Welan Gum by Ethanol Precipitation from Fermentation Broths

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作者研究了发酵液中威兰胶在乙醇溶剂作用下的沉淀行为,分析了乙醇质量浓度、pH值、盐离子种类和盐浓度等因素对威兰胶沉淀效果的影响,以及对沉淀物最终产物流变学性能的影响。确定威兰胶的最佳沉淀条件为:当添加2.5mol/LCaCl2溶液使威兰胶发酵液中的最终电荷浓度达到0.1mol/L,pH调至2.0,乙醇质量浓度为37.6g/dL时,可以有效沉淀分离得到威兰胶,收率为92.5%,并保持良好的流变学性能,比对照条件下的乙醇用量节省了73.3%。

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Welan gum,a kind of soluble extracellular heteropolysaccharide,is widely used in the oil exploration for enhancing oil recovery and construction due to its perfect stability and rheological properties.The precipitation characteristics of welan gum in the ethanol-water solution were investigated in this study.The influence of ethanol concentrations,pH,salt types and salt concentrations on welan precipitation and its rheological properties were investigated and the optimum parameters were listed as follows: 2.5 mol/L of CaCl2 was added until Ca2+ concentration reached at 0.1 mol/L,pH 2.0 and 37.6% of ethanol concentration in the bulk mixture.Welan gum was effectively precipitated and separated from the fermentation broth and the recovery yield achieved at 92.5%.Moreover,the rheological properties of the welan gum were kept robust,and 73.3% ethanol dosage was economized compared to the control experiments.

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曹花,季新跃,詹晓北,蒋芸,郑志永,姬彬,吴剑荣.发酵液中威兰胶的乙醇沉淀行为[J].食品与生物技术学报,2012,31(3):259-265.

CAO Hua, JI Xin-yue, ZHAN Xiao-bei, JIANG Yun, ZHENG Zhi-yong, JI Bin, WU Jian-rong. Recovery Characteristics of Welan Gum by Ethanol Precipitation from Fermentation Broths[J]. Journal of Food Science and Biotechnology,2012,31(3):259-265.

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