杜梨(Pyrus betulifolia Bge.)多糖提取工艺及其清除自由基活性

doi:10.3969/j.issn.1673-1689.2012.03.009

首页 > 过刊浏览>2012年第31卷第3期 >276-282. DOI:10.3969/j.issn.1673-1689.2012.03.009

杜梨(Pyrus betulifolia Bge.)多糖提取工艺及其清除自由基活性
DOI:
                        10.3969/j.issn.1673-1689.2012.03.009
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:国家自然科学基金项目(31070724);长江学者和创新团队发展计划项目(IRT0944)

Extracting Technology and Free Radical Scavenging Activity of Polysaccharide from Pyrus betulifolia Bge.Fruits

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

通过L9(33)正交试验获得杜梨(Pyrus betulaefolia Bge.)多糖最佳提取工艺。在运用紫外光谱、红外光谱及高效液相色谱等方法研究杜梨多糖(PBP)理化性质的基础上,进一步评价了PBP对羟自由基(.OH)、超氧阴离子自由基(O2-.)和1,1-二苯基-2-苦基肼(DPPH)自由基的清除活性。结果表明,在料液比为1∶15,90℃下提取5h的最优条件下,PBP提取率达到18.65%。多角激光光散射仪分析表明,PBP分子质量为392.9kD。单糖组成分析表明,PBP是由阿拉伯糖、半乳糖醛酸、鼠李糖、半乳糖和葡萄糖组成,摩尔比为49.54∶28.68∶8.81∶8.45∶4.54。PBP不仅具有清除羟自由基和超氧阴离子自由基活性,对DPPH自由基的清除活性更强。

Abstract:

The optimum extraction technology of polysaccharides from Pyrus betulaefolia was acquired by L9（33） orthogonal experiment design method.Physicochemical properties of Pyrus betulaefolia polysaccharide（PBP） were analyzed by ultraviolet spectra,infrared spectroscopy,and high performance liquid chromatography.The scavenging activities of hydroxyl free radicals,superoxide anion free radicals and DPPH free radicals were further evaluated.The results showed that the best condition was acquired when the ratio of solid to liquid was 1∶15 and extracting at 90 ℃ for 5 hours,and highest extraction yield of PBP was 18.65%.The molecular mass of PBP was 392.9 kD by multi-angle laser light scattering instrument.The monosaccharides of PBP were consisted of arabinose,galacturonic acid,rhamnose,galactose and glucose,with a molar ratio of 49.54∶28.68∶8.81∶8.45∶4.54,respectively.PBP not only showed obvious scavenging effect on hydroxyl and super oxygen-anion free radical,but also showed higher scavenging activity for DPPH free radical.

参考文献

相似文献

引证文献

引用本文

赵小亮,吕友晶,李苗苗,李广生,王玉峰,于广利.杜梨(Pyrus betulifolia Bge.)多糖提取工艺及其清除自由基活性[J].食品与生物技术学报,2012,31(3):276-282.

ZHAO Xiao-liang, LU You-jing, LI Miao-miao, LI Guang-sheng, WANG Yu-feng, YU Guang-li. Extracting Technology and Free Radical Scavenging Activity of Polysaccharide from Pyrus betulifolia Bge. Fruits[J]. Journal of Food Science and Biotechnology,2012,31(3):276-282.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2014-06-17
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码