菠萝茎蛋白酶的纯化及酶活力受超声处理影响的机理

doi:10.3969/j.issn.1673-1689.2012.05.005

首页 > 过刊浏览>2012年第31卷第5期 >473-478. DOI:10.3969/j.issn.1673-1689.2012.05.005

菠萝茎蛋白酶的纯化及酶活力受超声处理影响的机理
DOI:
                        10.3969/j.issn.1673-1689.2012.05.005
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:国家自然科学基金项目(10074016和10174021)

Purification of Stem Bromelain and Mechanism of Effect of Ultrasound on Its Catalysis Activity

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

对菠萝茎蛋白酶进行纯化和鉴定,并研究酶活力受超声处理影响的机理。采用羧甲基纤维素柱层析纯化、PAGE鉴定纯度,测定纯化的酶经超声波处理后的酶活性、动力学参数以及紫外和荧光光谱。柱层析分离出3个蛋白峰区域,其中第二蛋白峰经电泳鉴定纯度较高。酶经超声处理后Km变小,Vm也减小;紫外吸收光谱不变,而差示光谱出现了明显的正峰和负峰,荧光发射光谱基本不变。羧甲基纤维素柱层析对菠萝茎蛋白酶纯化效果较好,超声处理可能通过改变菠萝茎蛋白酶的构象从而改变酶活力。

Abstract:

To purify and identify stem bromelain for further study on its activity and mechanism after treated with ultrasound.Stem bromelain was purified and identified with carboxymethyl cellulose chromatography and polyacrylamide gel electrophoresis(PAGE) respectively.Enzyme activity,kinetic parameter,ultraviolet spectrum and fluorescence spectrum were studied on purified enzyme treated with ultrasound.Three single protein apex regions were separated with gradient elution.The ingredient with the highest activity in the second protein apex region was identified with PAGE,showing considerably high purity.After treatment with ultrasound,both Km and Vm decreased.No clear changes were observed in the ultraviolet absorption spectrum and the fluorescence emission spectrum while positive and negative peaks occurred in the differential ultraviolet spectrum.Conclusion: Considerably pure component of stem bromelain can be obtained after purified with carboxymethyl cellulose chromatography.It’s probably conformation change that results activity change after treatment of the ultrasound on stem bromelain.

参考文献

相似文献

引证文献

引用本文

陈小丽,黄卓烈.菠萝茎蛋白酶的纯化及酶活力受超声处理影响的机理[J].食品与生物技术学报,2012,31(5):473-478.

CHEN Xiao-li, HUANG Zhuo-lie. Purification of Stem Bromelain and Mechanism of Effect of Ultrasound on Its Catalysis Activity[J]. Journal of Food Science and Biotechnology,2012,31(5):473-478.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2014-06-17
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码