Abstract:In this manuscript,a halotolerant marine strain Aspergillus sp ZJUBE-1 possessed the production of endoglucanase and β-Gluase with high cellulase activities was isolated from the submarine mud sample of Zhejiang inshore,and identified as Aspergillus niger by two different methods of both the morphology and 18S rDNA gene sequences.The optimal medium compositions for liquid-state fermentation for cellulase production by strain ZJUBE-1 were determined and listed as follows: wheat bran 2.5%,NH4Cl or(NH4)H2PO4 1%,NaCl 3~ 3.6%.Under the condition of shaking culture for 7 days,at 37 ℃,the initial medium pH 6.0 and rotating speed 180 r/min,the maximum activities of carboxymethyl cellulase activity(CMCase),β-glucosidases(β-Gluase) and filter paper activity(FPA) were respectively reached at 5.3 U/mL-1,5.4 U/mL-1 and 0.75 U/mL,respectively.Moreover,the result showed that the strain still has cellulase productive ability,even the NaCl concentration exceed 24%.The studies on enzymatic properties showed that the optimal reaction temperature for CMCase,β-Gluase and FPA was the same temperature at 65 ℃,but the optimal pH value of three enzyme production were different,corresponding to 4.0,5.0,5.0,respectively.It was also found that even on the condition of 2~12% NaCl(β-Gluase is about in 2~16%),the production of three cellulase keep higher activities.These above-mentioned results indicated that the strain ZJUBE-1 has an extensive applications under high salinity environment in many fields,such as wastewater treatment,textile industry,HYPERLINK "http://dict.cnki.net/dict_result.aspx?searchword=%e6%b5%b7%e4%ba%a7%e5%93%81%e5%8a%a0%e5%b7%a5&tjType=sentence&style=&t=marine+products+processing" marine products processing and the utility of seaweed for energy source for its high salt-tolerant property in the near future.
