Abstract:To investigate the antioxidant effects in vitro and in vivo of Inonotus obliquus total flavonoids(TFIO),the radical scavenging capacity of TFIO and its inhibiting effect on lipoxidase activity were evaluated by the DPPH· model and the lipoxidase system,respectively.Meanwhile,the inhibitory action on hemolysis of mice RBC(red blood cell) by H2O2-induced was detected by spectrophotometry method,the effects in vitro and in vivo of TFIO on the formation of MDA(malondialdehyde) and ROS(reactive oxygen species) in mice liver were detected by the reagent kits.The results showed that in vitro,4.00 mg/mL TFIO showed 76.77% scavenging rate on DPPH·radical,81.73% inhibiting rate on lipoxygenase,83.33% inhibitory rate on mice RBC hemolysis by H2O2-induced,36.73%(non-induced),20.34%(Fe2+-induced) and 48.59%(H2O2-induced) inhibiting rates on MDA formation in mice liver homogenate,respectively,it also significantly increased the anti-ROS unit of mice liver homogenate to 50.75 U/mL(P<0.01,vs control group).In vivo,the MDA value reduced significantly(P<0.01,vs control group) and inhibiting rates of MDA formation reached 48.52% and 58.52% in mice liver after they were continuously treated by intra-peritoneal injected with 0.5 and 2.0 mg/mL TFIO(0.04 mL/(g·d),11 d),respectively.Therefore,it can be clearly seen that TFIO have strong antioxidant effect in vitro and in vivo.
