大麦芽多糖的降血糖活性及结构解析

doi:10.3969/j.issn.1673-1689.2012.10.013

首页 > 过刊浏览>2012年第31卷第10期 >1087-1092. DOI:10.3969/j.issn.1673-1689.2012.10.013

大麦芽多糖的降血糖活性及结构解析
DOI:
                        10.3969/j.issn.1673-1689.2012.10.013
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:国家“十一五”科技支撑计划项目(2007BAD83B02)

Hypoglycemic Effect and Structural Determination of Polysaccharides from Barley Malt

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

用四氧嘧啶糖尿病小鼠模型,考察大麦芽粗多糖的降糖作用,并通过DEAE-cellulose、HW-55F、Sephacryl S-400柱对有降血糖活性的组分进行分离。采用苯酚-硫酸法、薄层色谱法检测多糖纯度,通过单糖组成分析和1H-NMR、13C-NMR、135DEPT-NMR检测对多糖初级结构进行表征。结果表明:大麦芽粗多糖具有降低小鼠空腹血糖作用,对其餐后血糖效果不显著;粗多糖经DEAE-cellulose柱分离得到中性多糖和酸性多糖两个部分,其中水洗中性多糖经过HW-55F、Sephacryl S-400柱层析得到大麦芽多糖BPⅠ和BPⅡ。BPⅠ结构由呋喃葡萄糖组成,以1→3键相连,α和β构型交替出现;BPⅡ为半乳糖分子以1→4键相连形成主链,另有一个半乳糖分子连在主链糖分子的6-位上,形成侧链。其中,BPⅡ在大麦芽中首次报道。

Abstract:

Purpose: To investigate the separation method and the structure of polysaccharides with hypoglycemic effect from barley malt(BP).Methods: Alloxan-induced diabetic mice model was built,in which the hypoglycemic effect of polysaccharides from barley malt were determined.The polysaccharides from barley malt was purified to homogeneity through DEAE-cellulose,HW-55F and Sephacryl S-400 column chromatography.Meanwhile,the purity identification was analyzed by phenol-sulfuric method and thin layer chromatography.The structure was investigated using sugar composition analysis and 1H-NMR,13C-NMR,135DEPT-NMR analysis.Results: The polysaccharides can decrease fasting plasma glucose of the diabetes mice efficiently,and showed no significant effect on postprandial plasma glucose.Neutral polysaccharides(NBP)and acidic polysaccharides was isolated by DEAE-cellulose.BPⅠ and BPⅡ were further isolated from NBP by HW-55F and Sephacryl S-400.BPⅠconsists only of glucofuranose units joined by 1→3 bond,and both of α and β-configuration appear alternately.The main chain of BPⅡ is galactoses linked by 1→4 bond,and the galactose,forming lateral chain,is linked to C-6 of galactose in the main chain.BPⅡ is reported for the first time from barley malt.

参考文献

相似文献

引证文献

引用本文

杨延超,徐德平.大麦芽多糖的降血糖活性及结构解析[J].食品与生物技术学报,2012,31(10):1087-1092.

YANG Yan-chao, XU De-ping. Hypoglycemic Effect and Structural Determination of Polysaccharides from Barley Malt[J]. Journal of Food Science and Biotechnology,2012,31(10):1087-1092.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2014-06-17
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码