林蛙皮胶原蛋白肽酶解工艺数学建模及抗氧化作用的研究
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吉林省中小企业创新基金项目(20106042)


Mathematical Modeling for the Optimum Enzymatic Hydrolysis of Rana Skin Collagen Peptide and Its Antioxidant Research
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    摘要:

    采用数理统计和数据拟合分析方法对林蛙皮胶原蛋白肽(LWT)酶解过程中的影响因素进行优化,建立了LWT酶解过程中影响因素与水解度关系的数学模型,验证了水解度的最佳值(峰值)。采用聚丙烯酰胺凝胶电泳方法证明LWT的相对分子质量在3 500以下,HPLC表明其纯度较高。研究了LWT对溴代苯小鼠肝损伤的抗氧化作用,结果表明:LWT具有显著降低小鼠肝组织中MDA质量分数和显著升高小鼠肝组织中SOD活力的作用,具有抗氧化作用。

    Abstract:

    The influencing factors in the enzyme solution process of Rana skin collagen peptide(LWT) were optimized by using the mathematical statistics and data fitting analysis method.And the relationship between the influencing factors and hydrolyzing degree in the enzyme solution process of LWT was established.And the best value hydrolysis degree(peak) was forecasted.Used polyacrylamide gel electrophoresis method to determine the molecular weight of LWT which was under 3 500.High performance liquid chromatogram showed that the purity was in the high level.In the antioxidant effect of LWT on liver damage of bromobenzene mice were studied.The results showed that LWT have the remarkable effect on reducing MDA content and the obvious effect on rising SOD vitality in mice liver tissue and have antioxidant effect.

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邱芳萍,王长周,季晓枫.林蛙皮胶原蛋白肽酶解工艺数学建模及抗氧化作用的研究[J].食品与生物技术学报,2013,32(1):89-93.

QIU Fang-ping, WANG Chang-zhou, JI Xiao-feng. Mathematical Modeling for the Optimum Enzymatic Hydrolysis of Rana Skin Collagen Peptide and Its Antioxidant Research[J]. Journal of Food Science and Biotechnology,2013,32(1):89-93.

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  • 在线发布日期: 2014-06-17
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