响应曲面法优化玉竹水溶性多糖提取及体外抗氧化研究

doi:10.3969/j.issn.1673-1689.2013.03.011

首页 > 过刊浏览>2013年第32卷第3期 >298-306. DOI:10.3969/j.issn.1673-1689.2013.03.011

响应曲面法优化玉竹水溶性多糖提取及体外抗氧化研究
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                        10.3969/j.issn.1673-1689.2013.03.011
                    
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基金项目:中央高校基本科研业务费专项资金资助项目(GK200902042);陕西省自然科学基金项目(2003B08,2006B18);陕西师范大学勤助科研创新基金项目(QZZD11017)

Optimization of Water-Soluble Polysaccharides Extraction from Rhizome of Polygonatum odoratum Using Response Surface Methodology and Its Antioxidant Activities in Vitro

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摘要:

采用响应曲面法优化玉竹(Polygonatum odoratum(Mill.)Druce)水溶性多糖(POP)提取的工艺条件。在单因素试验基础上选取液料比、提取温度和提取时间3个关键影响因子,以POP提取率为响应值,依据回归分析确定POP最佳提取条件为:液料比为35∶1 mL/g,提取温度为88.35℃,提取时间为2.24 h,POP的提取率理论预测值为8.47%。在修正条件下,POP的实际提取率可达(8.46±0.02)%。POP对DPPH.、.OH和O2-.自由基均有一定的清除活性,相比之下对H2O2清除活性尤其明显,而且POP清除H2O2的IC50值为0.541 mg/mL,小于VC的IC50值(0.901 mg/mL)。

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A Box-Behnken design was used to optimize the extraction conditions of water-soluble polysaccharides(POP) from Polygonatum odoratum(Mill.) Druce.Three factors such as extraction time(h),extraction temperature(℃) and ratio of water to raw material were investigated.The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods.The optimum extraction conditions were listed as follows:extraction time 2.24 h,temperature 88.35 ℃,and ratio of water to raw material 35∶1 mL/g,the predicted yield of polysaccharides extracted was 8.47%.Under the optimum conditions,the mean extraction yield of polysaccharides was(8.46±0.02).The antioxidant in vitro results showed that the inhibition effects of POP on 2,2-diphenyl-2-picrylhydrazyl free radical,hydroxyl free radical and superoxide anion radical with IC50 values were 0.646,10.029,0.241 mg/mL,respectively,which were higher than 0.048,0.238,0.078 mg/mL of ascorbic acid,respectively.POP showed significant inhibitory effects on hydrogen peroxide with IC50 value of 0.541 mg/mL,which was lower than 0.901 mg/mL of ascorbic acid.

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杨颖,孙文武,周晨,何天龙,刘楠,朱苏骐,邵红军,段玉峰.响应曲面法优化玉竹水溶性多糖提取及体外抗氧化研究[J].食品与生物技术学报,2013,32(3):298-306.

YANG Ying, SUN Wen-wu, ZHOU Chen, HE Tian-long, LIU Nan, ZHU Su-qi, SHAO Hong-jun, DUAN Yu-feng. Optimization of Water-Soluble Polysaccharides Extraction from Rhizome of Polygonatum odoratum Using Response Surface Methodology and Its Antioxidant Activities in Vitro[J]. Journal of Food Science and Biotechnology,2013,32(3):298-306.

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