红旱莲总黄酮对黄嘌呤氧化酶抑制作用及抗氧化研究

doi:10.3969/j.issn.1673-1689.2013.04.003

首页 > 过刊浏览>2013年第32卷第4期 >353-357. DOI:10.3969/j.issn.1673-1689.2013.04.003

红旱莲总黄酮对黄嘌呤氧化酶抑制作用及抗氧化研究
DOI:
                        10.3969/j.issn.1673-1689.2013.04.003
                    
作者:
                        
                        
                    
作者单位:
作者简介:
通讯作者:
中图分类号:
基金项目:国家自然科学基金项目(31060210);江西省自然科学基金项目(20114BAB204019);江西省教育厅科技项目(GJJ11287)

Inhibitory Effect on Xanthine Oxidase and Antioxidant Activity of Total Flavonoids From Hypericum Ascyron L.

Author:

Affiliation:

Fund Project:

摘要

图/表

访问统计

参考文献

相似文献

引证文献

资源附件

文章评论

摘要:

通过抑制率及抑制动力学的测定,研究红旱莲总黄酮(HAF)对黄嘌呤氧化酶(XOD)的抑制作用及抑制机理。利用对羟基自由基清除能力、总抗氧化能力和总还原能力的测定,评估了红旱莲总黄酮体外抗氧化能力。结果表明,红旱莲总黄酮是一种可逆的竞争型XOD抑制剂,红旱莲总黄酮对XOD的抑制呈现浓度依赖关系,其半抑制质量浓度(IC50)为11.73μg/mL,抑制常数Ki为0.61μg/mL。红旱莲总黄酮有较好的羟基自由基清除能力(IC50=0.135μg/mL)及明显的总还原能力和总抗氧化能力。红旱莲黄酮可作为潜在的抗氧化剂和预防高尿酸血症的药物或膳食功能因子。

Abstract:

The inhibitory effect on xanthine oxidase(XOD) and its inhibitory mechanism of total flavonoids from Hypericum Ascyron L.(HAF) were investigated through the testing of inhibition rate and inhibition kinetics.The antioxidant activities of the flavonoids were evaluated in vitro by scavenging capability of hydroxyl free radical,total reductive ability and total antioxidant capability.The result showed that the flavonoids were reversible and competitive inhibitor,and their inhibitory effect on XOD showed a dosage-dependent manner.The half inhibition concentration(IC50) and inhibition constant(Ki) were obtained to be 11.73 μg/mL and 0.61 μg/mL,respectively.The antioxidant assay indicated that total flavonoids from Hypericum Ascyron L.possessed a considerable hydroxyl radicals scavenging activity(IC50=0.135 mg/mL),evident total reductive ability and total antioxidant capacity,which should be further investigated and utilized as a potential antioxidant and protective drug of hyperuricemia or dietary function factors.

参考文献

相似文献

引证文献

引用本文

周佳,张国文,胡明明.红旱莲总黄酮对黄嘌呤氧化酶抑制作用及抗氧化研究[J].食品与生物技术学报,2013,32(4):353-357.

ZHOU Jia, ZHANG Guo-wen, HU Ming-ming. Inhibitory Effect on Xanthine Oxidase and Antioxidant Activity of Total Flavonoids From Hypericum Ascyron L.[J]. Journal of Food Science and Biotechnology,2013,32(4):353-357.

复制

文章指标

点击次数:
下载次数:
HTML阅读次数:
引用次数:

历史

收稿日期:
最后修改日期:
录用日期:
在线发布日期: 2014-06-17
出版日期:

地址：江苏省无锡市蠡湖大道1800号邮政编码：214122

电话：0510-85913526 电子邮件：xbbjb@jiangnan.edu.cn

技术支持：北京勤云科技发展有限公司

引用本文

分享

文章指标

历史

微信公众号二维码

手机版网站二维码