不同皂苷质量分数速溶芦笋粉改善睡眠作用

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不同皂苷质量分数速溶芦笋粉改善睡眠作用
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基金项目:公益性行业(农业)科研专项经费项目(201303079);中央高校基本科研业务费专项资金项目(JUSRP111A35,JUSRP21115);江苏高校优势学科建设工程项目;秦皇岛市科学技术研究与发展计划项目(20121107001)

Study of the Instant Asparagus Powder with Different Content of Saponins on the Sleep Improvement Effect in Mice

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作者旨在研究不同皂苷质量分数速溶芦笋粉对小鼠改善睡眠作用的影响。18～22 g健康雄性昆明种小鼠按体质量随机分组,每天经口灌胃不同剂量皂苷含量为15%的速溶芦笋粉A和皂苷含量为50%的速溶芦笋粉B以及阳性对照物(酸枣仁皂苷),观察小鼠直接睡眠作用,连续给予受试物30 d后,进行戊巴比妥钠阈下剂量催眠试验、巴比妥钠睡眠潜伏期试验、延长戊巴比妥钠睡眠时间试验。结果表明:速溶芦笋粉A和速溶芦笋粉B均具有协同戊巴比妥钠阈下剂量催眠作用、能显著延长戊巴比妥钠睡眠时间;与空白组比较,速溶芦笋粉A缩短巴比妥钠睡眠潜伏期差异显著,而速溶芦笋粉B缩短巴比妥钠睡眠潜伏期的差异不显著,但在实验剂量范围内,其睡眠潜伏期呈逐渐缩短趋势。速溶芦笋粉A和速溶芦笋粉B均无直接改善睡眠作用。因此速溶芦笋粉A和B均具有改善睡眠作用,且速溶芦笋粉A对小鼠的睡眠改善作用较速溶芦笋粉B显著。

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The sleep improvement effects of instant asparagus powder with different content of saponins on mice were studied in this paper.Methods:Healthy KM male mice with the body weight of 18～22g were divided into groups randomized and the direct sleep effect of the test material was observed.Then the mice were gavaged with instant asparagus powder A with 15% saponins,instant asparagus powder B with 50% saponins and positive control(Jujuboside) in different dosage every day,respectively.30 days later,the sleep-producing test with subthreshold dose of sodium pentobarbital,the incubation period test with barbital sodium and the sleep extending test with sodium pentobarbital were conducted.Results:both of the asparagus powder A and B have hypnotic sleep-producing effects under the threshold hypnotic dosage of pentobarbital sodium,and can significantly prolong sleep time induced by pentobarbital sodium;comparing with the control group,the difference of incubation periods with barbital sodium were significant for the asparagus powder A,not significant for the asparagus powder B,but the incubation periods have gradually shorten trend with the asparagus powder B in this experiment.Both of asparagus powder A and B have not function to improve sleep directly.Conclusion:Instant asparagus powder A and B can improve the sleeping quality of mice,and the asparagus powder A is more significant than asparagus powder B on sleep improvement effects in mice.

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马淑凤,王芳,李汉臣,王周平,黄云祥.不同皂苷质量分数速溶芦笋粉改善睡眠作用[J].食品与生物技术学报,2013,32(8):793-798.

MA Shu-feng, WANG Fang, LI Han-chen, WANG Zhou-ping, HUANG Yun-xiang. Study of the Instant Asparagus Powder with Different Content of Saponins on the Sleep Improvement Effect in Mice[J]. Journal of Food Science and Biotechnology,2013,32(8):793-798.

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收稿日期:2012-09-27
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在线发布日期: 2013-10-28
出版日期: 2013-08-25

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