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色氨酸对慢性应激小鼠行为的影响
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国家“十二五”科技支撑计划项目(2012BAD33B00);江南大学2011年大学生创新训练计划项目(1025210232113480)


Effect of Tryptophan on Behavior of Chronically Stressed Mice
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    摘要:

    研究色氨酸对慢性应激小鼠行为的影响及与下丘脑5-HT的关系。将60只小鼠随机分为空白组(NC)、慢性应激模型组(CUS)、慢性应激模型+高剂量色氨酸(CUS-H)、慢性应激模型+低色氨酸(CUS-L),对CUS、CUS-H、CUS-L建立慢性应激模型,同时分别对CUS-H、CUS-L组灌胃高、低剂量的色氨酸,建模第5周开始进行旷场实验、悬尾实验、糖水消耗实验、强迫游泳实验及水迷宫实验等行为学实验,测定行为学指标,并采用ELISA法检测下丘脑5-HT含量。CUS-H组旷场实验得分显著高于CUS组与NC组(p<0.05),悬尾实验静止时间与CUS与NC组相比极显著地缩短(p<0.01),CUS-H组糖水偏好程度极显著低于CUS组(p<0.01),其强迫游泳静止时间极显著低于NC组(p<0.01);灌胃色氨酸对应激动物的学习和记忆能力有一定增强作用,但作用不显著;灌胃色氨酸可极显著地提高下丘脑5-HT含量(p<0.01)。研究结果表明,灌胃色氨酸能促进下丘脑5-HT的合成并有效增强动物的抗应激能力。抗应激能力的增强可能与下丘脑5-HT含量的提高有关。

    Abstract:

    Experiments were conducted to evaluate the effect of tryptophan on behavioral reactivity of stressed mice and its relation with hypothalamic 5-hydroxy tryptamine(5-HT).60 mice were randomly divided into 4 groups:NC group(saline administration),CUS group(saline administration and chronic stress stimulation),CUS-H group(high-dose tryptophan administration and chronic stress stimulation) and CUS-L group(low-dose tryptophan administration and chronic stress stimulation).A chronic stress model was established to CUS,CUS-H and CUS-L group.Meanwhile,different levels of tryptophan were administrated to CUS-H and CUS-L group.From the fifth week of establishing the model,open-field test,tail-suspension test,sugar preference test,forced swimming test and water maze were measured,and the hypothalamic serotonin level was assayed.The results are as follows:Compared to CUS and NC group,a notable increase(p<0.05) in open-field test score and a marked decrease(p<0.01)in immobile time of tail suspension test of CUS-H group were discovered,the sugar preference of CUS-H group were also decreased compared to CUS group(p<0.01).Besides,the immobile time of force swimming test was lower than NC group(p <0.01);Learning and memorizing ability of stressed mice was indistinctively improved after tryptophan administration;The Hypothalamic 5-HT content was obviously increased(p<0.01) after tryptophan administration.The results suggested that administration of tryptophan increase the content of hypothalamic 5-HT and effectively reverse the variation of stress resistant capability of stressed mice.The strengthened stress resistant capability might be related to the enhanced level of hypothalamic 5-HT.

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李萌萌,崔晓,朱凌峰,刘航,彭彦,辛琳,代卉,朱建津.色氨酸对慢性应激小鼠行为的影响[J].食品与生物技术学报,2013,32(8):838-843.

LI Meng-meng, CUI Xiao, ZHU Ling-feng, LIU Hang, PENG Yan, XIN Lin, DAI Hui, ZHU Jian-jin. Effect of Tryptophan on Behavior of Chronically Stressed Mice[J]. Journal of Food Science and Biotechnology,2013,32(8):838-843.

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  • 收稿日期:2012-07-26
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  • 在线发布日期: 2013-10-28
  • 出版日期: 2013-08-25

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