石榴皮多酚对脂变L-02肝细胞HMG-CoA还原酶mRNA表达的影响

doi:10.3969/j.issn.1673-1689.2013.09.010

首页 > 过刊浏览>2013年第32卷第9期 >957-961. DOI:10.3969/j.issn.1673-1689.2013.09.010

石榴皮多酚对脂变L-02肝细胞HMG-CoA还原酶mRNA表达的影响
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                        10.3969/j.issn.1673-1689.2013.09.010
                    
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基金项目:国家自然科学基金项目(31171677)

Effects of Pomegranate Peel Polyphenols(PPPs) on the Expression of HMG-CoA Reductase mRNA of Human L-02 Hepatocyte

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研究了石榴皮多酚纯化物、安石榴苷和石榴鞣花酸对脂变L-02肝细胞胆固醇合成限速酶HMG-CoA还原酶mRNA表达的影响;采用MTT法筛选石榴皮多酚适宜浓度;体积分数50%胎牛血清的RPMI-1640培养基与L-02肝细胞孵育48 h建立脂肪变性肝细胞模型;实验分为正常组、模型组和治疗组,治疗组加入不同浓度的受施物,继续培养48 h,采用RT-PCR法检测不同受施物对脂变L-02肝细胞HMG-CoA还原酶mRNA表达的影响;结果显示:石榴皮多酚均能呈剂量依赖性地减弱脂变L-02肝细胞mRNA表达,且以100μg/mL的安石榴苷标品抑制作用最强;表明石榴皮多酚降肝细胞总胆固醇作用是通过降低HMG-CoA还原酶mRNA表达实现的,安石榴苷是石榴皮多酚中降血脂的主要活性形式。

Abstract:

The objective of this manuscript is to study the effects of Pomegranate Peel Polyphenols,Punicalagine and ellagic acid on the expression of HMG-CoA reductase mRNA of human L-02hepatocyte. The model of steatotic L-02 hepatocyte was firstly established by cultured with 50%(V/V) fetal bovine serum for 48 hours,and the appropriate concentrations of PPPs were detected by MTT assay. Then the experiments were divided into three groups:normal group、model group and therapy group,different contents of PPPs is added to the therapy group. After 48 h cultured,the mRNA expression level of HMG-CoA reductase was detected by RT-PCR after 48 h cultured. It was found that the HMG-CoA reductase mRNA expression level was significantly inhibited by PPPs treatment groups,especially for the case of 100 μg/mL Punicalagin group. From the aboved results,the possible mechanisms of PPPs decreasing the intracellur cholestol are related to downregulation of HMG-CoA reductase mRNA’s expression,Punicalagin was the key component in PPPs on lipid-lowering.

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梁俊,李建科,刘永峰,张华峰,马倩倩,赵伟.石榴皮多酚对脂变L-02肝细胞HMG-CoA还原酶mRNA表达的影响[J].食品与生物技术学报,2013,32(9):957-961.

LIANG Jun, LI Jian-ke, LIU Yong-feng, ZHANG Hua-feng, MA Qian-qian, ZHAO Wei. Effects of Pomegranate Peel Polyphenols(PPPs) on the Expression of HMG-CoA Reductase mRNA of Human L-02 Hepatocyte[J]. Journal of Food Science and Biotechnology,2013,32(9):957-961.

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