桦褐孔菌多糖IOP3a体内抗肿瘤活性及其机制

doi:10.3969/j.issn.1673-1689.2013.09.015

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桦褐孔菌多糖IOP3a体内抗肿瘤活性及其机制
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                        10.3969/j.issn.1673-1689.2013.09.015
                    
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基金项目:2012年江苏省高校自然科学研究项目(12KJB550001);苏州市2011年应用基础研究计划(农业)项目(SYN201104)

Studies on Anti-Tumor Activity in Vivo and Its Mechanism of A Polysaccharide Named IOP3a From Inonotus obliquus

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通过建立裸鼠淋巴瘤细胞Jurkat模型,对桦褐孔菌多糖IOP3a的体内抗肿瘤活性及其机制进行了研究。实验结果表明,IOP3a高、中剂量组瘤重与阴性对照组相比均有显著性差异(p<0.05),肿瘤抑制率分别为69.28%和57.62%,具有明显的剂量-效应关系。随着剂量的增加,脾脏指数有逐步增加的趋势,具有升高裸鼠WBC和淋巴细胞的功能,同时对巨噬细胞产生的细胞因子TNF-α和L-1β均有明显的促进作用。组织病理学观察表明,IOP3a可使肿瘤组织中呈现明显的肿瘤坏死灶,瘤组织出现坏死的现象。免疫组化表明,IOP3a通过促进肿瘤组织细胞中Bax蛋白表达、抑制Bcl-2表达起到抗肿瘤作用。

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The aim of this manuscript was to check the antitumor activity of IOP3a and to elucidate the anticancer mechanism in vivo with cancer nude mice transplanted jurkat tumor as model.. it was found that tumor weight of high-dose and middle-dose had significant differences(p<0.05),when compared with the control group. Inhibition rate of tumor was 69.28% and 57.62%,respectively,which had an excellent dose-response relationship. IOP3a could increase the spleen index,white blood cell and lymphocyte with the increase of dosage. IOP3a promoted generation of TNF-α and L-1β from macrophage of nude mouse obviously. The histopathology of tumors indicated that the tumor cells from the different IOP3a-treated groups had clear necrosis areas in tumor tissues.The anti-tumor mechanism of IOP3a in vivo was that IOP3a could improve the expression of tumor gene Bax and inhibit the the expression of tumor gene Bcl-2 with immuno-histochemistry.

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陈义勇,黄友如,刘晶晶,郑丽雪,徐兵.桦褐孔菌多糖IOP3a体内抗肿瘤活性及其机制[J].食品与生物技术学报,2013,32(9):983-988.

CHEN Yi-yong, HUANG You-ru, LIU Jing-jing, ZHENG Li-xue, XU Bing. Studies on Anti-Tumor Activity in Vivo and Its Mechanism of A Polysaccharide Named IOP3a From Inonotus obliquus[J]. Journal of Food Science and Biotechnology,2013,32(9):983-988.

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在线发布日期: 2014-06-17
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