Abstract:The protective effect of soy isoflavones and saponins on acute liver injury induced by acetaminophen(APAP) in mice was investigated in this manuscript. The mice were randomly assigned to the normal control,model control,soy isoflavone,saponin and bifendate(positive control) groups. Animals were treated once daily for 7 days. APAP were given intraperitoneally to the mice of groups,then the alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),albumin(ALB),inducible nitric oxide synthase(iNOS),nitric oxide(NO),lipid hydroperoxide(LOOH),malondialdehyde(MDA),glutathione(GSH),catalase(CAT),glutathione peroxidase(GSH-Px) and superoxide dismutase(SOD) were detected by the colorimetric method. The administration with soy isoflavones and saponins could(1) reduced the serum ALT,AST and ALP;(2) increased the serum ALB level;(3)decreased hepatic iNOS activity and NO level,(4) decreased the hepatic LOOH and MDA contents,and(5)increased the CAT,GPx,SOD activities and GSH level of liver in mice with acute liver injury. Those results demonstrated that soy isoflavones and saponins have protective effects on acute liver injury induced by APAP in mice, probably via anti-inflammatory and nti-oxidative activities.
